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蟾毒灵通过抑制结肠癌细胞的干性来逆转获得性耐药。

Bufalin reverses acquired drug resistance by inhibiting stemness in colorectal cancer cells.

作者信息

Sun Jian, Xu Ke, Qiu Yanyan, Gao Hong, Xu Jianhua, Tang Qingfeng, Yin Peihao

机构信息

Interventional Cancer Institute of Integrative Medicine and Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China.

出版信息

Oncol Rep. 2017 Sep;38(3):1420-1430. doi: 10.3892/or.2017.5826. Epub 2017 Jul 17.

DOI:10.3892/or.2017.5826
PMID:28731184
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5549034/
Abstract

Drug resistance is an obstacle to chemotherapy in tumor patients. Recent studies have shown that the high stemness of cancer cells may be induced by chemotherapeutic drugs, which is correlated with drug resistance. In the present study, we investigated the effects of bufalin on the stemness of colorectal cancer. We found that cisplatin could induce high stemness through the tumorsphere formation assay in vitro and in vivo in the colorectal cancer cell lines HCT116 and LoVo. In addition, cisplatin-treated tumorsphere cells showed drug‑resistant properties. These results suggested that acquired drug resistance induced by cisplatin in colorectal cancer cells occurred via high stemness. On assessing the effects of bufalin, a traditional Chinese medicine monomer, we found that it could reverse the high stemness and drug resistance induced by cisplatin in colorectal cancer. These findings suggest that bufalin plays an adjuvant role in colorectal cancer chemotherapy and may help reverse acquired drug resistance. These findings may aid in the development of new therapeutic strategies.

摘要

耐药性是肿瘤患者化疗的一个障碍。最近的研究表明，化疗药物可能诱导癌细胞的高干性，这与耐药性相关。在本研究中，我们研究了蟾毒灵对结直肠癌干性的影响。我们发现顺铂可通过体外和体内肿瘤球形成试验在结直肠癌细胞系HCT116和LoVo中诱导高干性。此外，顺铂处理的肿瘤球细胞表现出耐药特性。这些结果表明，顺铂在结直肠癌细胞中诱导的获得性耐药是通过高干性发生的。在评估中药单体蟾毒灵的作用时，我们发现它可以逆转顺铂在结直肠癌中诱导的高干性和耐药性。这些发现表明，蟾毒灵在结直肠癌化疗中起辅助作用，可能有助于逆转获得性耐药。这些发现可能有助于开发新的治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/4306ef99848a/OR-38-03-1420-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/7728cc87d287/OR-38-03-1420-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/38e693ce91e0/OR-38-03-1420-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/f56d76774f33/OR-38-03-1420-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/4a49d5224ed3/OR-38-03-1420-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/342e873a335a/OR-38-03-1420-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/90a72e49e5b7/OR-38-03-1420-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/4306ef99848a/OR-38-03-1420-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/7728cc87d287/OR-38-03-1420-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/38e693ce91e0/OR-38-03-1420-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/f56d76774f33/OR-38-03-1420-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/4a49d5224ed3/OR-38-03-1420-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/342e873a335a/OR-38-03-1420-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/90a72e49e5b7/OR-38-03-1420-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43fc/5549034/4306ef99848a/OR-38-03-1420-g06.jpg

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