Takayanagi I, Koike K, Hiruta T
J Pharm Pharmacol. 1986 Jun;38(6):476-8. doi: 10.1111/j.2042-7158.1986.tb04615.x.
Single cells were prepared from the guinea-pig taenia caecum and used for the study of drug-receptor interactions. The cells showed a graded response to carbachol, the dose response curve being shifted in a parallel fashion by atropine, indicating a competitive antagonism. The pA2-value of atropine was in agreement with that estimated using the intact tissue. [3H]QNB (quinuclidinyl benzilate) combined with the single cells, the Scatchard plot yielding a straight line. The dissociation constant of QNB was similar to values estimated in other membrane preparations. The apparent dissociation constants of cholinergic drugs estimated from inhibition of the specific binding of [3H]QNB (0.20 nM) to the single cells by the cholinergic drugs were also in agreement with the values in other membrane preparations. The results indicate that the single smooth muscle cells are useful for the study of drug-receptor interactions.
从豚鼠盲肠带制备单细胞，并用于药物-受体相互作用的研究。这些细胞对卡巴胆碱呈分级反应，剂量反应曲线被阿托品以平行方式移动，表明存在竞争性拮抗作用。阿托品的pA2值与使用完整组织估计的值一致。[3H]QNB（喹核醇基苯甲酸酯）与单细胞结合，Scatchard图呈直线。QNB的解离常数与在其他膜制剂中估计的值相似。通过胆碱能药物抑制[3H]QNB（0.20 nM）与单细胞的特异性结合而估计的胆碱能药物的表观解离常数也与其他膜制剂中的值一致。结果表明，单个平滑肌细胞可用于药物-受体相互作用的研究。
