诱导痰中可检测到白细胞介素-32γ的哮喘患者的特征

Characteristics of asthmatics with detectable IL-32γ in induced sputum.

作者信息

Kwon Jae-Woo, Chang Hun Soo, Heo Jeong-Seok, Bae Da-Jeong, Lee Jong-Uk, Jung Chang An, Son Ji-Hye, Park Jong Sook, Kim Soo Hyun, Min Kyung-Up, Park Choon-Sik

机构信息

Division of Allergy and Clinical Immunology, Kangwon National University School of Medicine, Chuncheon, South Korea.

Department of Interdisciplinary Program in Biomedical Science Major, Soonchunhyang Graduate School, Bucheon, South Korea.

出版信息

Respir Med. 2017 Aug;129:85-90. doi: 10.1016/j.rmed.2017.06.005. Epub 2017 Jun 6.

DOI:10.1016/j.rmed.2017.06.005

PMID:28732840

Abstract

BACKGROUND

Interleukin-32(IL-32)γ is a pro-inflammatory cytokine involved in the development and severity of chronic inflammatory diseases, but its role in asthma is unclear.

OBJECTIVE

This study was conducted to evaluate the relationship of IL-32γ levels in sputum with the severity of asthma.

METHODS

IL-32γ levels in the supernatant of induced sputum obtained from 89 patients with stable asthma were measured using a sandwich enzyme-linked immunosorbent assay (ELISA). The relationships between sputum IL-32γ levels and baseline forced expiratory volume in 1 s (FEV% pred.), inflammatory cell profiles in sputum, and annual frequency of asthma exacerbation were determined.

RESULTS

IL-32γ was detected in the sputum of 25 of 89 (28.1%) asthma patients, and the levels of sputum were negatively correlated with FEV% pred. (ρ = -0.312, p = 0.003). The annual exacerbation rate was significantly higher in this group than in the IL-32-negative group (n = 64) (p = 0.03). Sputum IL-32γ levels correlated well with the annual exacerbation rate (ρ = 0.261, p = 0.014), but there were no differences in the inflammatory cell profiles in the induced sputum of IL-32-positive and IL-32-negative patients.

CONCLUSION

The level of IL-32γ in induced sputum may be associated with asthma severity and related with higher risk of asthma exacerbation.

摘要

背景

白细胞介素-32（IL-32）γ是一种促炎细胞因子，参与慢性炎症性疾病的发生发展及严重程度，但它在哮喘中的作用尚不清楚。

目的

本研究旨在评估痰液中IL-32γ水平与哮喘严重程度的关系。

方法

采用夹心酶联免疫吸附测定（ELISA）法检测89例稳定期哮喘患者诱导痰上清液中IL-32γ水平。确定痰液中IL-32γ水平与基线第1秒用力呼气容积（FEV%预计值）、痰液中炎症细胞谱以及哮喘急性发作年频率之间的关系。

结果

89例哮喘患者中有25例（28.1%）痰液中检测到IL-32γ，痰液中IL-32γ水平与FEV%预计值呈负相关（ρ = -0.312，p = 0.003）。该组的年急性发作率显著高于IL-32阴性组（n = 64）（p = 0.03）。痰液中IL-32γ水平与年急性发作率密切相关（ρ = 0.261，p = 0.014），但IL-32阳性和IL-32阴性患者诱导痰中的炎症细胞谱无差异。