Institute of Chemicobiology and Functional Materials, Nanjing University of Science and Technology, 200 Xiao Ling Wei Street, Nanjing, Jiangsu Province, China.
Institute of Chemicobiology and Functional Materials, Nanjing University of Science and Technology, 200 Xiao Ling Wei Street, Nanjing, Jiangsu Province, China.
Carbohydr Polym. 2017 Oct 1;173:547-555. doi: 10.1016/j.carbpol.2017.06.036. Epub 2017 Jun 10.
In this study, the reinforcing effects of cellulose nanofibrils (CNFs) on poly (vinyl alcohol) (PVA) matrix were explored. And ethylene glycol was used to enhance the water content and phosphate buffer saline (PBS) absorbency. The morphological aspects of the hydrogel were studied by transmission electron microscope (TEM) and scanning electron microscopy (SEM). The presence of interactions, changes in crystallinity as well as thermal behaviour were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermogravimetry respectively. With the increase of CNFs concentration, the composite greatly improved its mechanical strength while maintaining remarkable ductility through tensile test results. The positive results of cell toxicity test suggested our porous hydrogel could provide ideal cell growth environment. This work revealed that CNFs hydrolysed by bacterial cellulose could perform as a perfect reinforcing agents which is of great interest in the fields of biotechnology and biomedicine with the potential values in cell culture, co-cultivation, as well as biomedical scaffold materials.
在这项研究中，探索了纤维素纳米纤维（CNFs）对聚乙烯醇（PVA）基体的增强作用。并使用乙二醇来提高水含量和磷酸盐缓冲盐水（PBS）的吸水性。通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）研究了水凝胶的形态。通过傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）和热重分析分别研究了相互作用的存在、结晶度的变化以及热行为。随着 CNFs 浓度的增加，通过拉伸试验结果，复合材料大大提高了其机械强度，同时保持了显著的延展性。细胞毒性试验的阳性结果表明，我们的多孔水凝胶可以为细胞提供理想的生长环境。这项工作表明，由细菌纤维素水解得到的 CNFs 可以作为一种理想的增强剂，在生物技术和生物医学领域具有很大的应用价值，在细胞培养、共培养以及生物医学支架材料方面具有潜在的应用价值。
