Polymer Laboratory, School of Chemistry, College of Science, University of Tehran, PO Box 14155 6455, Tehran, Iran.
Polymer Laboratory, School of Chemistry, College of Science, University of Tehran, PO Box 14155 6455, Tehran, Iran.
Ultrason Sonochem. 2017 Nov;39:144-152. doi: 10.1016/j.ultsonch.2017.04.025. Epub 2017 Apr 20.
pH-responsive magnetic carriers at the nanoscale are one of the most important agents for the targeted treatment of cancer. In this study, FeO nanoparticles were prepared by co-precipitation method and functionalized with three types of PEG using ultrasound waves. PEGlated particles were modified with chitosan shell through ultrasound-assisted double emulsion method. The prepared material which was used as a pH responsive carrier for pinpointed 5-FU delivery. The chemico-physical properties of prepared nanoparticles have been investigated. Results demonstrated that pure FeO had a mean diameter of 20nm with the regular spherical shape which was increased after modification step depending on the type of PEG. 5-FU loading properties and releasing behaviors studies in different pHs which showed that 5-FU can be efficiently loaded in the FeO@Cs-PEG. Also, in the case of release, the amount of 5-FU released at pH=5.8 is noticeably higher compared to the released amount at pH=7.4 in all three samples at any distinct time. For instance at pH=7.4, 27% of the 5-FU was released from the FeO@Cs-PEG2 during 48h; as the pH decreases to 5.8, the cumulative amount of 5-FU released enhanced to 52%. The in vitro MTT assay results demonstrated that the cell viability decreases in all synthesized nanoparticles as the pH medium of MCF-7 culture became to 5.8. For example, cell viability of FeO@Cs-sPEG decreased from 44±2% to 36±1.9% at a concentration of 5 (μg/ml) as the pH varied from 7.4 to 5.8.
纳米级 pH 响应磁性载体是癌症靶向治疗的最重要试剂之一。在这项研究中，通过共沉淀法制备了 FeO 纳米粒子，并通过超声波用三种类型的 PEG 对其进行功能化。通过超声辅助的双重乳液法，用壳聚糖对 PEGlated 颗粒进行修饰。所制备的材料被用作定点递送 5-FU 的 pH 响应载体。研究了所制备的纳米粒子的理化性质。结果表明，纯 FeO 的平均粒径为 20nm，具有规则的球形形状，在修饰步骤后，根据 PEG 的类型而增加。在不同 pH 值下进行了 5-FU 负载性能和释放行为的研究，结果表明 5-FU 可以有效地负载在 FeO@Cs-PEG 中。此外，在释放方面，在任何特定时间，在所有三种样品中，在 pH=5.8 时释放的 5-FU 量明显高于在 pH=7.4 时的释放量。例如，在 pH=7.4 时，FeO@Cs-PEG2 在 48h 内释放了 27%的 5-FU；当 pH 降低至 5.8 时，释放的 5-FU 累积量增加至 52%。体外 MTT 测定结果表明，随着 MCF-7 培养物的 pH 介质变为 5.8，所有合成的纳米粒子中的细胞活力都降低。例如，当 pH 从 7.4 变化到 5.8 时，FeO@Cs-sPEG 的细胞活力从 44±2%降低到 36±1.9%，浓度为 5（μg/ml）。
