96孔板形式的转化
Transformation of in a 96-Well Format.
作者信息
Roguev Assen, Xu Jiewei, Krogan Nevan J
机构信息
Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518
Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518.
出版信息
Cold Spring Harb Protoc. 2018 Jan 2;2018(1):pdb.prot091942. doi: 10.1101/pdb.prot091942.
PMID:28733397
Abstract
This protocol describes chemical transformation of with linear DNA in a 96-well format. This procedure has been successfully used for large-scale strain construction in fission yeast.
摘要
本方案描述了在96孔板中用线性DNA对[具体内容缺失]进行化学转化的方法。该程序已成功用于裂殖酵母的大规模菌株构建。
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