裂殖酵母减数分裂的同步诱导

Synchronous Induction of Meiosis in the Fission Yeast .

作者信息

Yamashita Akira, Sakuno Takeshi, Watanabe Yoshinori, Yamamoto Masayuki

机构信息

Laboratory of Cell Responses, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan;

Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Aichi 444-8585, Japan.

出版信息

Cold Spring Harb Protoc. 2017 Sep 1;2017(9):pdb.prot091777. doi: 10.1101/pdb.prot091777.

DOI:10.1101/pdb.prot091777

PMID:28733399

Abstract

In fission yeast initiation of meiosis is repressed by Pat1 kinase. This protocol describes how ectopic inactivation of the temperature-sensitive Pat1-114 kinase in G-arrested / diploid cells carrying induces a highly synchronized commitment to and execution of meiosis. Haploid or diploid -114 mutants without can also be used for convenience, although less synchrony may be attained compared with induction using true diploids. An essentially identical protocol can be used for induction via inhibition of genetically sensitized Pat1 kinase by ATP analogs.

摘要

在裂殖酵母中，减数分裂的起始受到Pat1激酶的抑制。本实验方案描述了在携带[具体内容缺失]的G期停滞的单倍体/二倍体细胞中，温度敏感型Pat1-114激酶的异位失活如何诱导高度同步的减数分裂启动和进行。为方便起见，也可使用不携带[具体内容缺失]的单倍体或二倍体-114突变体，不过与使用真正的二倍体诱导相比，同步性可能会稍差。通过ATP类似物抑制基因敏感型Pat1激酶来进行诱导时，可使用基本相同的实验方案。