Departamento de Física Aplicada, Facultad de Ciencias, Univesidad de Granada, Campus Fuente Nueva s/n., Granada, 18071, Spain.
Adv Colloid Interface Sci. 2017 Sep;247:533-542. doi: 10.1016/j.cis.2017.07.015. Epub 2017 Jul 15.
In this review classical studies on insoluble liquid monolayers formed by proteins are examined and compared. It has been focused the attention on the information that it is possible to obtain from the π-a isotherms recorded by compression of the monolayers. In recent decades new techniques have developed, mainly microscopy, that provide valuable information on the behavior and structure of fluid films. However, frequently the data are difficult to interpret and require a previous thermodynamic study of them on the basis of the surface tension (or surface pressure) as a function of the molecular area measurement. The main aim of this paper is to underline that surface balance type of Langmuir is a powerful technique since it enables to obtain information at molecular level from a macroscopic analysis. Notably, this information is revealed very interesting when it comes to studying protein films. From this point of view it has been reviewed the study methods and results for four proteins.
在这篇综述中，考察和比较了蛋白质形成的不溶性液体单分子层的经典研究。本文主要关注通过压缩单分子层记录的 π-a 等温线可以获得的信息。在过去的几十年中，新技术得到了发展，主要是显微镜技术，为流体膜的行为和结构提供了有价值的信息。然而，这些数据往往难以解释，需要在基于表面张力（或表面压力）作为分子面积测量的函数的热力学研究的基础上对其进行解释。本文的主要目的是强调表面天平型 Langmuir 是一种强大的技术，因为它能够从宏观分析中获得分子水平的信息。值得注意的是，当涉及到蛋白质膜的研究时，这些信息变得非常有趣。从这个角度出发，综述了四种蛋白质的研究方法和结果。
