Nsayef Muslim D Sahira, Abbas Dham Ziyad, J Mohammed D Nadheer
Department of Biology, College of Science, AL-Mustansiryiah University, Baghdad, Iraq.
Department of Physics, College of Science, AL-Mustansiryiah University, Baghdad, Iraq.
Microb Pathog. 2017 Sep;110:484-493. doi: 10.1016/j.micpath.2017.07.024. Epub 2017 Jul 19.
Fourteen isolates of Serratia marcescens were collected from patients suffering from septicemia. All theseisolates revealed different levels in tannase production. Tannase was partially purified from Serratia marcescens b9 by precipitation method at 70% saturation of ammonium sulfate. Au, Pt, SnO and SiO nanoparticles were prepared by laser ablation and examined by transmission electron microscopy (TEM), X-ray diffraction pattern and UV-Visible absorption spectroscopy. Conjugation of SiO nanoparticles to tannase by feeding and pulses methods were prepared and characterized by TEM, X-ray diffraction pattern and UV-Visible spectrum. SiO nanoparticles conjugated partially purified tannase by feeding showed the higher effectiveness and higher significant level against all tested UTI causing in comparison with ciprofloxacin antibiotic, SiO nanoparticles alone, partially purified tannase alone and partially purified tannase by pulses. So that we can conclude that feeding method was the best method for enhancement partially purified tannase activity to maximum level thus SiO nanoparticles conjugated partially purified tannase may be a useful antibacterial agent for the treatment of urinary tract infection.
从败血症患者中收集了14株粘质沙雷氏菌分离株。所有这些分离株显示出不同水平的鞣酸酶产生。通过在硫酸铵70%饱和度下沉淀法从粘质沙雷氏菌b9中部分纯化鞣酸酶。通过激光烧蚀制备金、铂、二氧化锡和二氧化硅纳米颗粒，并通过透射电子显微镜（TEM）、X射线衍射图谱和紫外可见吸收光谱进行检测。通过进料和脉冲法制备了二氧化硅纳米颗粒与鞣酸酶的缀合物，并通过TEM、X射线衍射图谱和紫外可见光谱进行表征。与环丙沙星抗生素、单独的二氧化硅纳米颗粒、单独的部分纯化鞣酸酶以及通过脉冲法制备的部分纯化鞣酸酶相比，通过进料法制备的二氧化硅纳米颗粒缀合部分纯化鞣酸酶对所有测试的引起尿路感染的菌株显示出更高的有效性和更高的显著水平。因此我们可以得出结论，进料法是将部分纯化鞣酸酶活性提高到最大水平的最佳方法，因此二氧化硅纳米颗粒缀合部分纯化鞣酸酶可能是治疗尿路感染的一种有用的抗菌剂。
