Latha M, Sumathi M, Manikandan R, Arumugam A, Prabhu N M
Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India.
Department of Zoology, University of Madras, Guindy campus, Chennai 600 025, India.
Microb Pathog. 2015 May;82:43-9. doi: 10.1016/j.micpath.2015.03.008. Epub 2015 Mar 20.
In the present investigation, we described the green synthesis of silver nanoparticles using plant leaf extract of Hemidesmus indicus. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM images proved that the synthesized silver nanoparticles were spherical in shape with an average particle size of 25.24 nm. To evaluate antibacterial efficacy, bacteria was isolated from poultry gut and subjected to 16S rRNA characterization and confirmed as Shigella sonnei. The in vitro antibacterial efficacy of synthesized silver nanoparticles was studied by agar bioassay, well diffusion and confocal laser scanning microscopy (CLSM) assay. The H. indicus mediated synthesis of silver nanoparticles shows rapid synthesis and higher inhibitory activity (34 ± 0.2 mm) against isolated bacteria S. sonnei at 40 μg/ml.
在本研究中，我们描述了使用印度牛弥菜植物叶提取物绿色合成银纳米颗粒的过程。通过紫外可见光谱、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、透射电子显微镜（TEM）和能量色散X射线光谱（EDX）对合成的银纳米颗粒进行了表征。TEM图像证明合成的银纳米颗粒呈球形，平均粒径为25.24 nm。为了评估抗菌效果，从家禽肠道分离出细菌，并进行16S rRNA表征，确认为宋内志贺菌。通过琼脂生物测定法、孔扩散法和共聚焦激光扫描显微镜（CLSM）测定法研究了合成银纳米颗粒的体外抗菌效果。印度牛弥菜介导的银纳米颗粒合成显示出快速合成以及在40μg/ml时对分离出的宋内志贺菌具有更高的抑制活性（34±0.2 mm）。
