Muir C F, Priestnall S L, Hibbert A, Brown C, Garden O A, Scase T
Bridge Pathology Ltd, 637 Gloucester Rd, Bristol, UK; Pathobiology and Population Sciences, Royal Veterinary College, Hawkshead Lane, Hatfield, Hertfordshire, UK.
Pathobiology and Population Sciences, Royal Veterinary College, Hawkshead Lane, Hatfield, Hertfordshire, UK.
J Comp Pathol. 2017 Jul;157(1):15-22. doi: 10.1016/j.jcpa.2017.03.010. Epub 2017 Jun 6.
Diffuse large B-cell lymphoma (DLBCL) is the most common type of canine lymphoma and survival times are currently <1 year. Manipulation of the tumour microenvironment, of which the regulatory T cell (Treg) is a principal player, represents a potentially exciting way to curb the rapid proliferation of neoplastic cells. Tregs, characterized by the stable expression of the transcription factor FoxP3, suppress innate and adaptive arms of the immune response and represent a potential therapeutic target within neoplastic lymph nodes. This retrospective study explored the hypothesis that Tregs promote the proliferation of neoplastic large B cells, employing immunohistochemistry to assess both FoxP3 and Ki67 expression within canine lymph nodes. Fifty-seven biopsy samples of canine nodal DLBCL were examined. There were significantly fewer FoxP3 cells in lymph nodes effaced by DLBCL than in reactive lymph nodes (27 versus 369 cells/mm; Mann-Whitney U = 16, P = 0.011). There was no relationship between the number of intratumoural FoxP3 cells and neoplastic cell proliferation (Spearman's rank r = 0.058, P = 0.670, 95% confidence interval). The results of this study show that FoxP3 cells are reduced in lymph nodes effaced by DLBCL and that this change is unrelated to the expression of Ki67. This study also describes a robust digital method to standardize cell counts and facilitate future comparative studies.
弥漫性大B细胞淋巴瘤（DLBCL）是犬类淋巴瘤最常见的类型，目前其存活时间小于1年。肿瘤微环境的调控（其中调节性T细胞（Treg）是主要参与者）代表了一种抑制肿瘤细胞快速增殖的潜在有效方法。Treg以转录因子FoxP3的稳定表达为特征，可抑制免疫反应的先天性和适应性分支，并代表肿瘤淋巴结内的一个潜在治疗靶点。这项回顾性研究探讨了Treg促进肿瘤性大B细胞增殖的假说，采用免疫组织化学方法评估犬类淋巴结内FoxP3和Ki67的表达。检查了57例犬淋巴结DLBCL的活检样本。与反应性淋巴结相比，DLBCL侵犯的淋巴结中FoxP3细胞明显减少（分别为27个细胞/mm和369个细胞/mm；Mann-Whitney U = 16，P = 0.011）。肿瘤内FoxP3细胞数量与肿瘤细胞增殖之间无相关性（Spearman等级r = 0.058，P = 0.670，95%置信区间）。本研究结果表明，DLBCL侵犯的淋巴结中FoxP3细胞减少，且这种变化与Ki67的表达无关。本研究还描述了一种可靠的数字方法，用于标准化细胞计数并促进未来的比较研究。
