Harada Takumi, Tomii Naoki, Manago Shota, Kobayashi Etsuko, Sakuma Ichiro
Department of Bioengineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Department of Precision Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Biomed Opt Express. 2017 Mar 24;8(4):2339-2358. doi: 10.1364/BOE.8.002339. eCollection 2017 Apr 1.
To measure the activity of tissue at the microscopic level, laminar optical tomography (LOT), which is a microscopic form of diffuse optical tomography, has been developed. However, obtaining sufficient recording speed to determine rapidly changing dynamic activity remains major challenges. For a high frame rate of the reconstructed data, we here propose a new LOT method using compressed sensing theory, called compressive laminar optical tomography (CLOT), in which novel digital micromirror device-based illumination and data reduction in a single reconstruction are applied. In the simulation experiments, the reconstructed volumetric images of the action potentials that were acquired from 5 measured images with random pattern featured a wave border at least to a depth of 2.5 mm. Consequently, it was shown that CLOT has potential for over 200 fps required for the cardiac electrophysiological phenomena.
为了在微观层面测量组织的活性，已经开发出了层流光学层析成像（LOT），它是漫射光学层析成像的一种微观形式。然而，要获得足够的记录速度以确定快速变化的动态活性仍然是主要挑战。为了实现重建数据的高帧率，我们在此提出一种使用压缩感知理论的新LOT方法，称为压缩层流光学层析成像（CLOT），其中在单次重建中应用了基于新型数字微镜器件的照明和数据缩减。在模拟实验中，从具有随机模式的5幅测量图像中获取的动作电位的重建体积图像在至少2.5毫米的深度处具有波边界。因此，结果表明CLOT有潜力实现心脏电生理现象所需的超过200帧/秒的帧率。
