Amrita Centre for Industrial Research & Innovation, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Ettimadai, Coimbatore 641112, India.
School of Biotechnology, Amrita University, Kollam, Kerala 690525, India.
Chem Commun (Camb). 2017 Aug 11;53(62):8802-8805. doi: 10.1039/c7cc05339h. Epub 2017 Jul 24.
We report the application of a chemodosimeter 'turn on' fluorescent probe for detecting endogenous HS formation in cancer cells. Mito-HS showed a bathochromic shift in the UV-vis-absorption spectrum from 355 nm to 395 nm in the presence of HS. Furthermore, it showed an ∼43-fold fluorescence enhancement at λ = 450 nm in the presence of HS (200 μM). The cancer cell-specific fluorescence imaging reveals that Mito-HS has the ability to distinguish cancer cells from normal cells based on the level of endogenous HS formation. In due course, Mito-HS would be a powerful cancer biomarker based on its ability to estimate endogenous HS formation in living cells.
我们报告了一种化学计量 '开启' 荧光探针在检测癌细胞内源性 HS 形成中的应用。在 HS 的存在下，Mito-HS 在紫外-可见吸收光谱中从 355nm 到 395nm 发生红移。此外，在 HS（200μM）存在下，在 λ = 450nm 处显示出约 43 倍的荧光增强。癌细胞特异性荧光成像显示，Mito-HS 能够根据内源性 HS 形成的水平区分癌细胞和正常细胞。因此，Mito-HS 将成为一种基于其在活细胞中估计内源性 HS 形成能力的强大癌症生物标志物。
