Hwang S Y, Kingsbury W D, Hall N M, Jakas D R, Dunn G L, Gilvarg C
Anal Biochem. 1986 May 1;154(2):552-8. doi: 10.1016/0003-2697(86)90028-x.
The peptide mimetic L-phenylalanyl-L-3-thiaphenylalanine has been shown to facilitate a sensitive and simple determination of leucine aminopeptidase. A colorimetric assay, employing Ellman's reagent to detect the thiophenol released upon hydrolysis of the dipeptide, has been developed. Under the experimental conditions employed the substrate has a Km of 0.054 mM and a kcat of 5800 min-1 and can distinguish sharply between leucine aminopeptidase and aminopeptidase M.
肽模拟物L-苯丙氨酰-L-3-硫代苯丙氨酸已被证明有助于灵敏且简便地测定亮氨酸氨肽酶。一种采用埃尔曼试剂检测二肽水解时释放的苯硫酚的比色测定法已被开发出来。在所采用的实验条件下，该底物的米氏常数（Km）为0.054 mM，催化常数（kcat）为5800 min⁻¹，并且能够清晰地区分亮氨酸氨肽酶和氨肽酶M。
