School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.
Bioresour Technol. 2017 Nov;243:893-897. doi: 10.1016/j.biortech.2017.07.067. Epub 2017 Jul 14.
Proteomics and metabolomics analysis has become a powerful tool for characterization of microbial ability for fixation of Carbon dioxide. Bacterial community of palaeoproterozoic metasediments was enriched in the shake flask culture in the presence of NaHCO. One of the isolate showed resistance to NaHCO (100mM) and was identified as Serratia sp. ISTD04 by 16S rRNA sequence analysis. Carbon dioxide fixing ability of the bacterium was established by carbonic anhydrase enzyme assay along with proteomic analysis by LC-MS/MS. In proteomic analysis 96 proteins were identified out of these 6 protein involved in carbon dioxide fixation, 11 in fatty acid metabolism, indicating the carbon dioxide fixing potency of bacterium along with production of biofuel. GC-MS analysis revealed that hydrocarbons and FAMEs produced by bacteria within the range of C-C and C-C respectively. Presence of 59% saturated and 41% unsaturated organic compounds, make it a better fuel composition.
蛋白质组学和代谢组学分析已成为表征微生物固定二氧化碳能力的有力工具。古元古代变质砂岩中的细菌群落在含有 NaHCO 的摇瓶培养中得到了富集。其中一个分离株对 NaHCO（100mM）表现出抗性，并通过 16S rRNA 序列分析鉴定为 Serratia sp. ISTD04。通过碳酸酐酶酶测定和 LC-MS/MS 进行的蛋白质组学分析，确定了细菌的二氧化碳固定能力。在蛋白质组学分析中，共鉴定出 96 种蛋白质，其中 6 种与二氧化碳固定有关，11 种与脂肪酸代谢有关，表明了细菌的二氧化碳固定能力以及生物燃料的生产。GC-MS 分析表明，细菌在 C-C 和 C-C 范围内分别产生碳氢化合物和 FAMEs。存在 59%的饱和和 41%的不饱和有机化合物，使其成为更好的燃料成分。
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