School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
Bioresour Technol. 2014 Aug;165:201-4. doi: 10.1016/j.biortech.2014.02.075. Epub 2014 Mar 1.
A CO2 sequestering bacterial strain, Serratia sp. ISTD04, that produces a significant amount of extracellular lipids was isolated from marble mine rocks. (14)C labeling analysis revealed that the rate of assimilation of CO2 by the strain is 0.756×10(-9)μmolCO2fixedcell(-1)h(-1). It was found to produce 466mg/l of extracellular lipid which was characterized using (1)H NMR. After transesterification of lipids, the total saturated and unsaturated FAME was found to be 51% and 49% respectively. The major FAME contained in the biodiesel were palmitic acid methyl ester (C16:0), oleic acid methyl ester (C18:1) and 10-nonadecenoic acid methyl ester (C19:1). Biodiesel produced by Serratia sp. ISTD04 is balanced in terms of FAME composition of good quality. It also contained higher proportion of oleic acid (35%) which makes it suitable for utilization in existing engines. Thus, the strain can be harnessed commercially to sequester CO2 into biodiesel.
从大理石矿岩中分离出一株能够产生大量胞外脂质的固碳细菌菌株 Serratia sp. ISTD04。(14)C 标记分析表明，该菌株同化 CO2 的速率为 0.756×10(-9)μmolCO2fixedcell(-1)h(-1)。结果发现该菌株能够产生 466mg/L 的胞外脂质，并用 (1)H NMR 对其进行了表征。对脂质进行酯交换后，发现总饱和和不饱和 FAME 分别为 51%和 49%。生物柴油中主要的 FAME 包含棕榈酸甲酯（C16:0）、油酸甲酯（C18:1）和 10-十九烯酸甲酯（C19:1）。Serratia sp. ISTD04 生产的生物柴油在 FAME 组成方面具有良好的平衡，其油酸含量较高（35%），这使其适合在现有发动机中使用。因此，该菌株可以被商业化利用，将 CO2 固定为生物柴油。
