Jolivet-Gougeon Anne, Helsens Nicolas, Renard Elise, Tamanai-Shacoori Zohreh, Bonnaure-Mallet Martine
Université de Rennes, INSERM U 1241, INRA, Nutrition Metabolisms and Cancer, 2 avenue du Professeur Léon Bernard, 35043 Rennes, France; CHU Pontchaillou, 2 rue Henri Le Guilloux, 35033 Rennes Cedex 9, France.
Université de Rennes, INSERM U 1241, INRA, Nutrition Metabolisms and Cancer, 2 avenue du Professeur Léon Bernard, 35043 Rennes, France.
Anaerobe. 2017 Dec;48:89-93. doi: 10.1016/j.anaerobe.2017.07.003. Epub 2017 Jul 21.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for rapid identification of cfxA PCR positive and negative Capnocytophaga strains. Colonies were grown on blood agar, incubated anaerobically at 37 °C for 48 h, and were then evaluated by MALDI-TOF MS and 16S rRNA gene sequencing. Both methods identified all colonies to the genus level. The MALDI-TOF MS method gave the same result, at the species level, as 16S rRNA gene sequencing for 41/53 Capnocytophaga sp. strains (77.4%), but the limit of this technique was the absence of some species (C. leadbetteri, C. AHN) in the Biotyper-Bruker database used in this study. Distinction between the cefotaxime resistant and susceptible strains was unsuccessful using the MALDI-TOF MS method. This technique had low discriminatory power to rapidly detect beta-lactamase-producing Capnocytophaga strains in clinical samples. However, the results from a score-oriented dendrogram confirmed MALDI-TOF MS is a rapid, inexpensive, and reliable method for Capnocytophaga species identification. Enrichment of the reference database used (Biotyper) will improve future results.
评估了基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）用于快速鉴定cfxA PCR阳性和阴性二氧化碳嗜纤维菌菌株的效果。将菌落接种于血琼脂平板上，在37℃厌氧培养48小时，然后通过MALDI-TOF MS和16S rRNA基因测序进行评估。两种方法均能将所有菌落鉴定到属水平。对于53株二氧化碳嗜纤维菌中的41株（77.4%），MALDI-TOF MS方法在种水平上与16S rRNA基因测序结果一致，但该技术的局限性在于本研究使用的Biotyper-Bruker数据库中缺少某些菌种（利德比特二氧化碳嗜纤维菌、AHN二氧化碳嗜纤维菌）。使用MALDI-TOF MS方法无法区分耐头孢噻肟和敏感菌株。该技术在快速检测临床样本中产生β-内酰胺酶的二氧化碳嗜纤维菌菌株方面鉴别能力较低。然而，基于评分的树状图结果证实MALDI-TOF MS是一种快速、廉价且可靠的二氧化碳嗜纤维菌菌种鉴定方法。丰富所用的参考数据库（Biotyper）将改善未来的结果。
