Pervaiz S, Brew K
Comp Biochem Physiol A Comp Physiol. 1986;84(2):357-60. doi: 10.1016/0300-9629(86)90629-8.
Milk samples from four individual bottlenose (Tursiops truncatus) and two Florida manatees (Trichechus manatus latirostris) of known lactation stages were analyzed for protein, carbohydrate and lipid composition, as well as for activity levels of alpha-lactalbumin, the regulatory protein of lactose synthase. The milk from both species had relatively high protein and lipid levels, as reported previously for other marine mammals. The major proportion of the lipid was in the form of triglycerides. Dolphin milk contained an average of 2.2% neutral sugars, which was essentially all in the form of lactose, as determined by several criteria. Manatee milk samples contained 0.6% of neutral sugars, and a larger proportion (about 2%) of amino sugars. Lactose was not detected by enzymatic assay or paper chromatography, but HPLC analysis indicated the presence of low levels of lactose together with two components that were tentatively identified as oligosaccharides. alpha-Lactalbumin activity, determined by assay with bovine galactosyltransferase, was found in both dolphin and manatee milk. The level in dolphin milk was comparable with that found in bovine and other milk, but the level in the manatee was less than 10% of that in the dolphin.
对来自四只处于已知泌乳阶段的宽吻海豚（瓶鼻海豚）和两只佛罗里达海牛（南美海牛指名亚种）的乳汁样本进行了蛋白质、碳水化合物和脂质成分分析，以及乳糖合酶的调节蛋白α-乳白蛋白活性水平的分析。如先前对其他海洋哺乳动物的报道一样，这两个物种的乳汁中蛋白质和脂质水平相对较高。脂质的主要部分以甘油三酯的形式存在。通过多种标准测定，海豚乳汁平均含有2.2%的中性糖，基本上全部为乳糖形式。海牛乳汁样本含有0.6%的中性糖和较大比例（约2%）的氨基糖。通过酶法测定或纸色谱法未检测到乳糖，但高效液相色谱分析表明存在低水平的乳糖以及两种初步鉴定为寡糖的成分。通过与牛半乳糖基转移酶测定发现，海豚和海牛的乳汁中均存在α-乳白蛋白活性。海豚乳汁中的水平与牛和其他乳汁中的水平相当，但海牛乳汁中的水平不到海豚乳汁中水平的10%。
