基于转谷氨酰胺酶的超灵敏纳米传感器，用于人类乳糜泻的早期检测。
Ultrasensitive transglutaminase based nanosensor for early detection of celiac disease in human.
机构信息
Shoolini University, Post Box No. 9, Head Post Office, Solan, H.P. 173212, India.
Shoolini University, Post Box No. 9, Head Post Office, Solan, H.P. 173212, India; CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi-110007, India.
出版信息
Int J Biol Macromol. 2017 Dec;105(Pt 1):905-911. doi: 10.1016/j.ijbiomac.2017.07.126. Epub 2017 Jul 21.
A GQD/PAMAM nanohybrid modified on gold nanoparticles (AuNP) embedded in multiwalled carbon nanotubes (MWCNT) based immunosensor was developed for detection of celiac disease in human. The AuNP embedded in MWCNT were linked with thiol groups of cysteine and amino groups were further coupled with carboxyl moieties of graphene quantum dots (GQDs) using EDC-NHS cross-linking chemistry. To enhance the sensitivity of the sensor, polyamidoamine (PAMAM) dendrimer was attached with GQDs through carbodiimide coupling. The tissue transglutaminase (tTG) antigen was immobilized on PAMAM to form nanoprobe for sensing specific anti-tissue transglutaminase (anti-tTG) antibodies (immunoglobin A isotype) in human serum. The electrochemical changes after specific binding of antibody with antigen was measured cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The modifications in sensor surface were characterized using FE-SEM. The sensor is highly specific to anti-tissue transglutaminase antibodies and showed negligible response to non-specific serum proteins. The sensitivity of the sensor was 1297.14μA/cm/pg with DPV and lower limit of detection of anti-tissue transglutaminase antibody was found 0.1fg per 6μl.
一种基于金纳米粒子（AuNP）嵌入多壁碳纳米管（MWCNT）的 GQD/PAMAM 纳米杂化体修饰的免疫传感器被开发用于检测人类的乳糜泻。嵌入 MWCNT 的 AuNP 通过半胱氨酸的巯基与 MWCNT 连接，然后通过 EDC-NHS 交联化学进一步与石墨烯量子点（GQDs）的羧基偶联。为了提高传感器的灵敏度，通过碳二亚胺偶联将聚酰胺-胺（PAMAM）树状大分子与 GQDs 连接。组织转谷氨酰胺酶（tTG）抗原固定在 PAMAM 上，形成纳米探针，用于检测人血清中特定的抗组织转谷氨酰胺酶（抗-tTG）抗体（免疫球蛋白 A 同种型）。特异性结合抗原后，通过循环伏安法（CV）和差分脉冲伏安法（DPV）测量电化学变化。通过 FE-SEM 对传感器表面的修饰进行了表征。该传感器对抗组织转谷氨酰胺酶抗体具有高度特异性，对非特异性血清蛋白几乎没有反应。使用 DPV 时，传感器的灵敏度为 1297.14μA/cm/pg，检测抗组织转谷氨酰胺酶抗体的下限为 6μl 时为 0.1fg。
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