荷瘤小鼠模型中NKG2DL表达与蛋白结合多糖K抗肿瘤作用的相关性

Correlation Between NKG2DL Expression and Antitumor Effect of Protein-bound Polysaccharide-K in Tumor-bearing Mouse Models.

作者信息

Konagai Ayano, Yoshimura Kiyoshi, Hazama Shouichi, Yamamoto Noboru, Aoki Kazunori, Ueno Tomio, Fujioka Masaki, Iijima Hiroko, Kato Mariko, Uchida Motoyuki, Wada Tsutomu, Inoue Moeko, Asao Tetsuhiko, Fuse Masanori, Wada Satoshi, Kuramasu Atsuo, Kamei Ryoji, Takeda Shigeru, Yamamoto Shigeru, Yoshino Shigefumi, Oka Masaaki, Nagano Hiroaki

机构信息

Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.

Pharmaceuticals & Agrochemicals Division, Kureha Corporation, Tokyo, Japan.

出版信息

Anticancer Res. 2017 Aug;37(8):4093-4101. doi: 10.21873/anticanres.11796.

DOI:10.21873/anticanres.11796

PMID:28739693

Abstract

BACKGROUND/AIM: We investigated the relationship between the expression of natural killer group 2, member D ligands (NKG2DLs) and the antitumor effects of protein-bound polysaccharide-K (PSK).

MATERIALS AND METHODS

PSK was administered to evaluate its effectiveness against tumor growth. The expression of Rae-1 and H60 were analyzed in multiple cell lines.

RESULTS

PSK showed the highest antitumor effects in mice implanted with cells expressing neither Rae-1 nor H60. PSK had little antitumor effect in mice implanted with cells expressing both Rae-1 and H60. A correlation between the expression of NKG2DLs and the antitumor effect of PSK was observed. After PSK administration, INF-γ production in CD8 T cells increased in mice with cells expressing neither Rae-1 nor H60, but did not change in mice implanted with cells expressing both Rae-1 and H60.

CONCLUSION

We demonstrated that the expression of NKG2DLs affects tumor immunity and the efficacy of immuno therapy in tumor-bearing mouse model.

摘要

背景/目的：我们研究了自然杀伤细胞2族D成员配体（NKG2DLs）的表达与蛋白结合多糖-K（PSK）的抗肿瘤作用之间的关系。

材料与方法

给予PSK以评估其对肿瘤生长的有效性。分析了多种细胞系中Rae-1和H60的表达。

结果

PSK在植入既不表达Rae-1也不表达H60的细胞的小鼠中显示出最高的抗肿瘤作用。PSK在植入同时表达Rae-1和H60的细胞的小鼠中几乎没有抗肿瘤作用。观察到NKG2DLs的表达与PSK的抗肿瘤作用之间存在相关性。给予PSK后，在植入既不表达Rae-1也不表达H60的细胞的小鼠中，CD8 T细胞中的INF-γ产生增加，但在植入同时表达Rae-1和H60的细胞的小鼠中没有变化。