一种用于重建长尿道缺损的、专门分泌LL37的细胞接种管状支架的临床前研究。

A Preclinical Study of Cell-seeded Tubularized Scaffolds Specially Secreting LL37 for Reconstruction of Long Urethral Defects.

作者信息

Li Yongwei, Wu Jitao, Feng Fan, Men Changping, Yang Diandong, Gao Zhenli, Zhu Zhe, Cui Yuanshan, Zhao Hongwei

机构信息

Department of Urology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China.

Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, U.S.A.

出版信息

Anticancer Res. 2017 Aug;37(8):4295-4301. doi: 10.21873/anticanres.11822.

DOI:10.21873/anticanres.11822

PMID:28739721

Abstract

AIM

We constructed a new artificial, long tubular acellular matrix, seeded with autologous progenitor cells transfected with the sequence to produce the antibiotic peptide LL37 and another two common seeding cells, which might be adopted for patients requiring repair of long segment of the urethra.

MATERIALS AND METHODS

Autologous endothelial progenitor cells transfected by lentiviral vectors expressing antibiotic peptide LL37, as well as urothelial and smooth muscle cells from New Zealand white male rabbits, were cultured and seeded onto preconfigured acellular collagen-based tubular matrices (3 cm in length). Artificial conduits were created again in New Zealand white male rabbits and, then, evaluated by immunohistochemistry after 8 weeks.

RESULTS

Cell-seeded tubularized collagen scaffolds were found to be effective in repairing long urethral defects, whereas scaffolds without cells led to poor tissue development and structures.

CONCLUSION

The artificial tissue engineered tubularized scaffolds combined with genetic methods resulted in vascularized autologous grafts, which may potentially be used for urethroplasty in patients requiring repair of a long segment of the urethra.

摘要