Gong Zhaohua, Chi Cheng, Huang Xiaojuan, Chu Hongjin, Wang Jiahui, Du Fengcai, Jiang Lixin, Chen Jian
Department of Oncology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China.
First Department of Chemotherapy, Weihai Municipal Hospital, Weihai, P.R. China.
Anticancer Res. 2017 Aug;37(8):4443-4447. doi: 10.21873/anticanres.11839.
To investigate the expression of cyclophilin A (CypA) in human hepatocellular carcinoma (HCC) and explore the effects of CypA on the cell cycle in HCC.
CypA expression was assessed by immunohistochemistry in 48 cases of HCC tissues and paired adjacent tissues. CypA plasmid was transfected into HCC cells and the cell cycle was analyzed.
Positivity for CypA was higher in HCC tissues than in adjacent tissues (79.1% vs. 12.5%, p<0.05). Positivity for CypA was significantly higher in stage III and IV HCC than in stage I and II (p<0.05). Elevated CypA induced an increase of the percentage of S-phase cells (from 34.79% to 42.14%) and a decrease of G-G phase cells (from 58.10% to 50.64%).
CypA is overexpressed in HCC and is associated with TNM stage. CypA also appears to promote the transition of the cell cycle from G to S phase.
研究亲环素A（CypA）在人肝细胞癌（HCC）中的表达情况，并探讨CypA对肝癌细胞周期的影响。
采用免疫组织化学法检测48例肝癌组织及配对的癌旁组织中CypA的表达。将CypA质粒转染至肝癌细胞中，并分析细胞周期。
肝癌组织中CypA的阳性率高于癌旁组织（79.1%对12.5%，p<0.05）。III期和IV期肝癌中CypA的阳性率显著高于I期和II期（p<0.05）。CypA表达升高导致S期细胞百分比增加（从34.79%增至42.14%），G-G期细胞百分比降低（从58.10%降至50.64%）。
CypA在肝癌中过度表达，且与TNM分期相关。CypA似乎还能促进细胞周期从G期向S期的转变。
