Tzelepis Georgios, Bejai Sarosh, Sattar Muhammad Naeem, Schwelm Arne, Ilbäck Jonas, Fogelqvist Johan, Dixelius Christina
Department of Plant Biology, Uppsala BioCenter, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, P.O. Box 7080, 75007, Uppsala, Sweden.
Department of Plant Virology, Institute of Agricultural Sciences (IAGS), University of the Punjab, Quaid-e-Azam Campus, Box. 54590, Lahore, Pakistan.
Arch Microbiol. 2017 Dec;199(10):1383-1389. doi: 10.1007/s00203-017-1412-z. Epub 2017 Jul 24.
Verticillium species are soilborne plant pathogens, responsible for big yield losses worldwide. Here, we report improved procedures to generate DNA from Verticillium species imbedded in farm soils. Using new genomic sequence information, primers for V. dahliae, V. albo-atrum, V. tricorpus, and V. longisporum were designed. In a survey of 429 samples from intensively farmed soil of two Swedish regions, only V. dahliae and V. longisporum were identified. A bias towards V. longisporum (40%) was seen in the south, whereas V. dahliae was more frequent in the western region (19%). Analyses of soil and leaf samples from 20 sugar beet fields, where foliar wilting had been observed, revealed V. dahliae DNA in all leaf and soil samples and V. longisporum in 18 soil samples, illustrating host choice and longevity of the V. longisporum microsclerotia. This study demonstrates the applicability of new molecular diagnostic tools that are important for growers of variable crops.
轮枝菌属是土壤传播的植物病原体，在全球范围内造成巨大的产量损失。在此，我们报告了从嵌入农田土壤中的轮枝菌属提取DNA的改进方法。利用新的基因组序列信息，设计了针对大丽轮枝菌、黑白轮枝菌、三孢轮枝菌和长孢轮枝菌的引物。在对瑞典两个地区集约化耕作土壤的429个样本进行的调查中，仅鉴定出大丽轮枝菌和长孢轮枝菌。在南部地区发现长孢轮枝菌存在偏向性（40%），而大丽轮枝菌在西部地区更为常见（19%）。对20个已观察到叶片枯萎的甜菜田的土壤和叶片样本进行分析，结果显示所有叶片和土壤样本中均存在大丽轮枝菌DNA，18个土壤样本中存在长孢轮枝菌DNA，这说明了长孢轮枝菌微菌核的寄主选择和存活时间。本研究证明了新的分子诊断工具对多种作物种植者的适用性。
