通过用人血清处理，溶组织内阿米巴HM1对补体溶解的敏感性丧失。
Loss of susceptibility to complement lysis in Entamoeba histolytica HM1 by treatment with human serum.
作者信息
Calderon J, Tovar R
出版信息
Immunology. 1986 Jul;58(3):467-71.
Abstract
Entamoeba histolytica HM1, exposed to a series of treatment with normal human serum (NHS), progressively lost susceptibility to complement lysis. Trophozoites were incubated daily with unheated or heat-inactivated NHS for 2 hr at 36 degrees, starting with 10% v/v serum and increasing by 5% every 3 days up to 40% NHS. Resistance to complement lysis was also obtained with two different HM1 clones but not with the low virulent strain HK9. Induction of resistance dependent on the number of NHS treatments, with a maximal 50% reduction occurring after 11 treatments. Susceptibility to complement-dependent lysis was regained 6 weeks after serum treatments were terminated, suggesting that resistance to lysis was an acquired rather than a genetic property.
摘要
溶组织内阿米巴HM1经一系列正常人血清（NHS）处理后，对补体溶解的敏感性逐渐丧失。滋养体每天在36℃下与未加热或热灭活的NHS孵育2小时，起始血清浓度为10%（体积/体积），每3天增加5%，直至40%NHS。两种不同的HM1克隆也获得了对补体溶解的抗性，但低毒力菌株HK9没有。抗性的诱导取决于NHS处理的次数，11次处理后最大降低50%。血清处理终止6周后，对补体依赖性溶解的敏感性恢复，这表明对溶解的抗性是后天获得的而非遗传特性。
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