Kong Rong-Mei, Zhang Xiaobin, Ding Lu, Yang Daoshan, Qu Fengli
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong, 273165, China.
Lab of Advanced Materials, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai, 200438, China.
Anal Bioanal Chem. 2017 Sep;409(24):5757-5765. doi: 10.1007/s00216-017-0519-z. Epub 2017 Jul 24.
Fluorescent light-up probes based on aggregation-induced emission (AIE)-active molecules have recently attracted great research interest due to the intelligent fluorescence activation mechanism and high sensitivity. In this work, an AIE-silica nanosphere (SiO NP)-based label-free fluorescent aptasensor for the sensitive "turn-on" detection of prostate-specific antigen (PSA) is reported for the first time. The positively charged amino-functionalized SiO NPs were used as efficient nanocapturer to electrostatically adsorb single-stranded PSA aptamer (PA) to form SiO NP-PA nanocomposite as well as adsorb negatively charged tetraphenylethylene derivative 3 (TPE3) to form AIE-SiO NP nanocomposite. The binding of the aptamer to the target PSA could induce a rigid aptamer conformation, resulting in the release of the PA away from the surface of SiO NPs. This made the AIE molecules TPE3 aggregate on the SiO NP surface and emit high fluorescence. With the advantages of simple design and rapid responses, the proposed aptasensor showed high sensitivity and selectivity for PSA with a detection limit of 0.5 ng/mL. The aptasensor was further applied in human serum samples with satisfactory results. Given its versatility, high selectivity, and sensitivity, the proposed method could be extended to other targets by varying the recognition probes. Graphical abstract An AIE-SiO NP-based label-free fluorescent aptasensor for the sensitive "turn-on" detection of PSA is reported for the first time.
基于聚集诱导发光（AIE）活性分子的荧光点亮探针，因其智能的荧光激活机制和高灵敏度，近来引起了极大的研究兴趣。在本工作中，首次报道了一种基于AIE-二氧化硅纳米球（SiO NP）的无标记荧光适配体传感器，用于灵敏的“开启”检测前列腺特异性抗原（PSA）。带正电荷的氨基功能化SiO NPs被用作高效的纳米捕获剂，以静电吸附单链PSA适配体（PA），形成SiO NP-PA纳米复合材料，同时吸附带负电荷的四苯乙烯衍生物3（TPE3），形成AIE-SiO NP纳米复合材料。适配体与目标PSA的结合可诱导适配体构象刚性化，导致PA从SiO NPs表面释放。这使得AIE分子TPE3在SiO NP表面聚集并发出高荧光。该适配体传感器具有设计简单、响应快速的优点，对PSA表现出高灵敏度和选择性，检测限为0.5 ng/mL。该适配体传感器进一步应用于人体血清样本，结果令人满意。鉴于其通用性、高选择性和灵敏度，通过改变识别探针，所提出的方法可扩展到其他目标。图形摘要首次报道了一种基于AIE-SiO NP的无标记荧光适配体传感器，用于灵敏的“开启”检测PSA。
