Wang Zhaojun, Liu Qinfu, Wang Xiaohong, Wang Yanbo, Zhang Jun, Zhou Wenjie, Yang Xiaojun
Department of Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China (Wang ZJ, Liu QF, Wang XH, Zhang J, Zhou WJ, Yang XJ); Department of Cerebrospinal Fluid Research, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China (Wang YB). Corresponding author: Yang Xiaojun, Email:
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2017 Jul;29(7):592-595. doi: 10.3760/cma.j.issn.2095-4352.2017.07.004.
To observe differential expressions of microRNA-127-5p (miR-127-5p) in bronchoalveolar lavage fluid (BALF) of patients with severe pneumonia and the value of miR-127-5p in the diagnosis of severe pneumonia.
Thirty severe pneumonia patients and 10 non-respiratory infection patients who needed mechanical ventilation after surgery admitted to Department of Critical Care Medicine of General Hospital of Ningxia Medical University from January to December in 2015 were enrolled, whose specimens of BALF were collected. The differential expressions of miRNA in BALF of patients in both groups were screened by miRNA chip technique to preliminarily establish miRNA differential expression profiles in BALF of severe pneumonia, and the miRNAs which were up-regulated and down-regulated were screened out. The expression levels of miR-127-5p were determined using a real-time fluorescent quantitative polymerase chain reaction (PCR). The value of miR-127-5p expression in the diagnosis of severe pneumonia was evaluated with receiver-operating characteristic curve (ROC).
All of the 40 patients were enrolled in the final analysis. Differential expression spectrum of miRNA in severe pneumonia patients was initially built, in which 40 miRNAs were up-regulated and 113 miRNAs were down-regulated. Compared with non-respiratory infection patients, the expressions of miR-127-5p were significantly lowered in severe pneumonia patients (2: 0.578±0.226 vs. 1.004±0.337) with statistical difference (t = 4.552, P = 0.000). ROC curve analysis showed that the area under ROC curve (AUC) of miR-127-5p for diagnosis of severe pneumonia was 0.855 [95% confidence interval (95%CI) = 0.721-0.989, P = 0.001], with the optimal sensitivity and specificity of 86.7% and 70.0% respectively with 0.840 as the critical value, and the positive likelihood ratio was 2.89, the negative likelihood ratio was 0.19.
miR-127-5p in BALF could be used as a new biomarker for the diagnosis of severe pneumonia.
观察重症肺炎患者支气管肺泡灌洗液（BALF）中微小RNA-127-5p（miR-127-5p）的差异表达情况及miR-127-5p在重症肺炎诊断中的价值。
选取2015年1月至12月在宁夏医科大学总医院重症医学科住院的30例重症肺炎患者及10例术后需机械通气的非呼吸道感染患者，采集其BALF标本。采用miRNA芯片技术筛选两组患者BALF中miRNA的差异表达，初步建立重症肺炎患者BALF中miRNA差异表达谱，筛选出上调和下调的miRNA。采用实时荧光定量聚合酶链反应（PCR）检测miR-127-5p的表达水平。用受试者工作特征曲线（ROC）评估miR-127-5p表达在重症肺炎诊断中的价值。
40例患者均纳入最终分析。初步构建了重症肺炎患者miRNA差异表达谱，其中40个miRNA上调，113个miRNA下调。与非呼吸道感染患者相比，重症肺炎患者miR-127-5p的表达明显降低（2：0.578±０.226 vs. 1.004±０.337），差异有统计学意义（t = 4.552，P = 0.000）。ROC曲线分析显示，miR-127-5p诊断重症肺炎的ROC曲线下面积（AUC）为0.855[95%置信区间（95%CI）= 0.721 - 0.989，P = 0.001]，以0.840为临界值时，最佳灵敏度和特异度分别为86.7%和70.0%，阳性似然比为2.89，阴性似然比为0.19。
BALF中的miR-127-5p可作为重症肺炎诊断的新型生物标志物。
