Shiroto Yuki, Terashima Shingo, Hosokawa Yoichiro, Oka Kyoko, Isokawa Keitaro, Tsuruga Eichi
Department of Radiation Science, Graduate School of Health Sciences, Hirosaki University, 66-1 Honcho, Hirosaki, Aomori 036-8564, Japan.
Section of Pediatric Dentistry, Department of Oral Growth & Development, Division of Clinical Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
Acta Histochem Cytochem. 2017 Jun 26;50(3):105-109. doi: 10.1267/ahc.16036. Epub 2017 Jun 14.
The ciliary zonules link the lens to the ciliary body in the eye, controlling the thickness of the lens for focusing through their characteristic elasticity. The ciliary zonules are composed of oxytalan fibers. Physiological or pathological damage to the ciliary zonules, including exposure to ultraviolet (UV)-A and UV-B components, can lead to lens dislocation. However, no studies have shown whether UV affects the ciliary zonule. Here, we assessed the effects of UV light on human nonpigmented ciliary epithelial cells (HNPCECs). HNPCECs were cultured for 4 weeks, and expression of fibrillin-1 and fibrillin-2 was confirmed. In control cultures (0 mJ/cm), some fibrillin-1-positive fibers were merged with fibrillin-2. After UV-A irradiation, the appearance of both fibrillin-1- and fibrillin-2-positive fibers was unchanged. However, after UV-B irradiation, fibrillin-1-positive fibers became thin at an irradiation level of 100 mJ/cm, and the fiber structure became amorphous at 150 mJ/cm. Fibrillin-2-positive fibers lost their continuity and disappeared after being exposed to 150 mJ/cm UV-B. UV-B irradiation did not affect cell viability, possibly because of the sensitivity of fibrillin-1 and fibrillin-2 to UV-B. Thus, dislocation of the lens with age may be attributable to cumulative exposure to UV-B.
睫状小带将眼睛中的晶状体与睫状体相连，通过其特有的弹性控制晶状体的厚度以实现聚焦。睫状小带由弹力纤维组成。睫状小带的生理或病理损伤，包括暴露于紫外线（UV）-A和UV-B成分，可导致晶状体脱位。然而，尚无研究表明紫外线是否会影响睫状小带。在此，我们评估了紫外线对人非色素睫状上皮细胞（HNPCECs）的影响。将HNPCECs培养4周，并确认原纤蛋白-1和原纤蛋白-2的表达。在对照培养物（0 mJ/cm）中，一些原纤蛋白-1阳性纤维与原纤蛋白-2融合。紫外线-A照射后，原纤蛋白-1和原纤蛋白-2阳性纤维的外观均未改变。然而，紫外线-B照射后，在100 mJ/cm的照射水平下，原纤蛋白-1阳性纤维变细，在150 mJ/cm时纤维结构变为无定形。原纤蛋白-2阳性纤维在暴露于150 mJ/cm紫外线-B后失去连续性并消失。紫外线-B照射不影响细胞活力，这可能是由于原纤蛋白-1和原纤蛋白-2对紫外线-B敏感。因此，晶状体随年龄的脱位可能归因于对紫外线-B的累积暴露。
