Fesahat Farzaneh, Montazeri Fatemeh, Sheikhha Mohammad Hasan, Saeedi Hojjatollah, Dehghani Firouzabadi Razieh, Kalantar Seyed Mehdi
Genetics Department, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Int J Reprod Biomed. 2017 May;15(5):297-304.
Selection of the best embryo for transfer is very important in assisted reproductive technology (ART). Using morphological assessment for this selection demonstrated that the correlation between embryo morphology and implantation potential is relatively weak. On the other hand, aneuploidy is a key genetic factor that can influence human reproductive success in ART.
The aim of this lab trial study was to evaluate the incidence of aneuploidies in five chromosomes in the morphologically high-quality embryos from young patients undergoing ART for sex selection.
A total of 97 high quality embryos from 23 women at the age of 37or younger years that had previously undergone preimplantation genetic screening for sex selection were included in this study. After washing, the slides of blastomeres from embryos of patients were reanalyzed by fluorescence in-situ hybridization for chromosomes 13, 18 and 21.
There was a significant rate of aneuploidy determination in the embryos using preimplantation genetic screening for both sex and three evaluated autosomal chromosomes compared to preimplantation genetic screening for only sex chromosomes (62.9% vs. 24.7%, p=0.000). The most frequent detected chromosomal aneuploidy was trisomy or monosomy of chromosome 13.
There is considerable numbers of chromosomal abnormalities in embryos generated in vitro which cause in vitro fertilization failure and it seems that morphological characterization of embryos is not a suitable method for choosing the embryos without these abnormalities.
在辅助生殖技术（ART）中，选择最佳胚胎进行移植非常重要。通过形态学评估进行这种选择表明，胚胎形态与着床潜力之间的相关性相对较弱。另一方面，非整倍体是一个关键的遗传因素，可影响ART中人类生殖的成功率。
本实验室试验研究的目的是评估接受ART进行性别选择的年轻患者形态学上高质量胚胎中五条染色体的非整倍体发生率。
本研究纳入了23名年龄在37岁及以下的女性的97个高质量胚胎，这些女性此前已接受过植入前遗传学性别筛选。洗涤后，通过荧光原位杂交对患者胚胎的卵裂球玻片进行重新分析，检测13、18和21号染色体。
与仅进行性染色体的植入前遗传学筛选相比，同时进行性别和三条评估常染色体的植入前遗传学筛选时，胚胎中非整倍体的检测率显著更高（62.9%对24.7%，p = 0.000）。最常检测到的染色体非整倍体是13号染色体三体或单体。
体外产生的胚胎中存在大量染色体异常，这会导致体外受精失败，而且胚胎的形态特征似乎不是选择无这些异常胚胎的合适方法。
