Hendler Adi, Medina Edgar M, Buchler Nicolas E, de Bruin Robertus A M, Aharoni Amir
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, 84105, Beersheba, Israel.
Department of Biology, Duke University, Durham, NC, USA.
Curr Genet. 2018 Feb;64(1):81-86. doi: 10.1007/s00294-017-0726-3. Epub 2017 Jul 25.
The G1-to-S cell cycle transition is promoted by the periodic expression of a large set of genes. In Saccharomyces cerevisiae G1/S gene expression is regulated by two transcription factor (TF) complexes, the MBF and SBF, which bind to specific DNA sequences, the MCB and SCB, respectively. Despite extensive research little is known regarding the evolution of the G1/S transcription regulation including the co-evolution of the DNA binding domains with their respective DNA binding sequences. We have recently examined the co-evolution of the G1/S TF specificity through the systematic generation and examination of chimeric Mbp1/Swi4 TFs containing different orthologue DNA binding domains in S. cerevisiae (Hendler et al. in PLoS Genet 13:e1006778. doi: 10.1371/journal.pgen.1006778 , 2017). Here, we review the co-evolution of G1/S transcriptional network and discuss the evolutionary dynamics and specificity of the MBF-MCB and SBF-SCB interactions in different fungal species.
一大组基因的周期性表达促进了细胞从G1期到S期的转变。在酿酒酵母中，G1/S基因的表达受两种转录因子（TF）复合物MBF和SBF调控，它们分别与特定的DNA序列MCB和SCB结合。尽管进行了广泛研究，但关于G1/S转录调控的进化，包括DNA结合结构域与其各自DNA结合序列的共同进化，我们了解得还很少。我们最近通过在酿酒酵母中系统地生成和检测含有不同直系同源DNA结合结构域的嵌合Mbp1/Swi4转录因子，研究了G1/S转录因子特异性的共同进化（亨德勒等人，《公共科学图书馆·遗传学》，第13卷，e1006778。doi:10.1371/journal.pgen.1006778，2017年）。在此，我们回顾了G1/S转录网络的共同进化，并讨论了不同真菌物种中MBF-MCB和SBF-SCB相互作用的进化动态和特异性。
