Crane Matthew M, Tsuchiya Mitsuhiro, Blue Ben W, Almazan Jared D, Chen Kenneth L, Duffy Siobhan R, Golubeva Alexandra, Grimm Annaiz M, Guard Alison M, Hill Shauna A, Huynh Ellen, Kelly Ryan M, Kiflezghi Michael, Kim Hyunsung D, Lee Mitchell, Lee Ting-I, Li Jiayi, Nguyen Bao M G, Whalen Riley M, Yeh Feng Y, McCormick Mark, Kennedy Brian K, Delaney Joe R, Kaeberlein Matt
Department of Pathology, University of Washington, Seattle, WA, USA.
Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
Transl Med Aging. 2019;3:104-108. doi: 10.1016/j.tma.2019.10.002. Epub 2019 Oct 31.
An increase in cell size with age is a characteristic feature of replicative aging in budding yeast. Deletion of the gene encoding Whi5 results in shortened duration of G1 and reduced cell size, and has been previously suggested to increase replicative lifespan. Upon careful analysis of multiple independently derived haploid and homozygous diploid mutants, we find no effect on lifespan, but we do confirm the reduction in cell size. We suggest that instead of antagonizing lifespan, the elongated G1 phase of the cell cycle during aging may actually play an important role in allowing aged cells time to repair accumulating DNA damage.
随着年龄增长细胞大小增加是芽殖酵母复制性衰老的一个特征。编码Whi5的基因缺失会导致G1期持续时间缩短和细胞大小减小，并且此前有人认为这会增加复制寿命。在对多个独立衍生的单倍体和纯合二倍体突变体进行仔细分析后，我们发现对寿命没有影响，但我们确实证实了细胞大小的减小。我们认为，衰老过程中细胞周期延长的G1期可能并非对抗寿命，而是实际上在让衰老细胞有时间修复积累的DNA损伤方面发挥重要作用。
