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牛苯乙醇胺N-甲基转移酶的完整核苷酸序列和推导的氨基酸序列：与大鼠酪氨酸羟化酶的部分氨基酸同源性

Complete nucleotide and deduced amino acid sequence of bovine phenylethanolamine N-methyltransferase: partial amino acid homology with rat tyrosine hydroxylase.

作者信息

Baetge E E, Suh Y H, Joh T H

出版信息

Proc Natl Acad Sci U S A. 1986 Aug;83(15):5454-8. doi: 10.1073/pnas.83.15.5454.

DOI:10.1073/pnas.83.15.5454
PMID:2874553
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC386305/
Abstract

We report here the isolation of a cDNA clone containing the full coding region of bovine phenylethanolamine N-methyltransferase (PNMTase, EC 2.1.1.28, S-adenosyl-L-methionine:phenylethanolamine N-methyltransferase). The complete nucleotide sequence of the cDNA has been determined, and the amino acid sequence of PNMTase deduced. Cultured cells transfected with an expression vector containing this cDNA produced high levels of PNMTase enzymatic activity. Antibodies specific for tyrosine hydroxylase [EC 1.14.16.2, tyrosine 3-monooxygenase; L-tyrosine, tetrahydrobiopterine: oxygen oxidoreductase (3-hydroxylating)], the first enzyme in the catecholamine pathway, possess a striking affinity for the PNMTase protein synthesized in vitro. Comparison of the deduced amino acid sequence of bovine PNMTase to rat tyrosine hydroxylase reveals that PNMTase shares significant homology with tyrosine hydroxylase and supports previous protein and immunological data suggesting that the catecholamine biosynthetic enzymes are structurally related.

摘要

我们在此报告一个包含牛苯乙醇胺N-甲基转移酶（PNMTase，EC 2.1.1.28，S-腺苷-L-甲硫氨酸：苯乙醇胺N-甲基转移酶）完整编码区的cDNA克隆的分离。已确定该cDNA的完整核苷酸序列，并推导了PNMTase的氨基酸序列。用含有此cDNA的表达载体转染的培养细胞产生了高水平的PNMTase酶活性。对儿茶酚胺途径中的第一种酶酪氨酸羟化酶[EC 1.14.16.2，酪氨酸3-单加氧酶；L-酪氨酸，四氢生物蝶呤：氧氧化还原酶（3-羟基化）]具有特异性的抗体，对体外合成的PNMTase蛋白具有显著的亲和力。将牛PNMTase推导的氨基酸序列与大鼠酪氨酸羟化酶进行比较，发现PNMTase与酪氨酸羟化酶具有显著的同源性，并支持先前的蛋白质和免疫学数据，表明儿茶酚胺生物合成酶在结构上相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb8c/386305/ea3d1ec1b5c9/pnas00319-0110-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb8c/386305/29450bbc15c9/pnas00319-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb8c/386305/180d5cdc4f99/pnas00319-0110-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb8c/386305/ea3d1ec1b5c9/pnas00319-0110-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb8c/386305/29450bbc15c9/pnas00319-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb8c/386305/180d5cdc4f99/pnas00319-0110-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb8c/386305/ea3d1ec1b5c9/pnas00319-0110-c.jpg

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