Bradley D E
Plasmid. 1986 Jul;16(1):63-71. doi: 10.1016/0147-619x(86)90080-6.
The conjugation system of the IncHI3 plasmid MIP233 was studied using a transfer-derepressed Tn5-insertion mutant. The conjugative pili of this plasmid were short pointed rods resembling rigid pili, with a well-defined modal length. Unlike plasmids with rigid pili, the MIP233 mutant mediated a surface + liquid conjugation system. The pili were serologically different from all known pilus types including H pili, and did not act as receptors for any known pilus-specific bacteriophage. They converted the surface conjugation system of RP4 to a surface + liquid one. Antiserum to pili of the mutant plasmid inhibited transfer of the wild-type plasmid MIP233, demonstrating that it contained only one transfer system.
使用转座抑制的Tn5插入突变体研究了IncHI3质粒MIP233的接合系统。该质粒的接合菌毛是短尖杆状，类似于刚性菌毛，具有明确的模态长度。与具有刚性菌毛的质粒不同，MIP233突变体介导了一种表面+液体接合系统。这些菌毛在血清学上与所有已知菌毛类型不同，包括H菌毛，并且不作为任何已知菌毛特异性噬菌体的受体。它们将RP4的表面接合系统转变为表面+液体接合系统。针对突变体质粒菌毛的抗血清抑制了野生型质粒MIP233的转移，表明它仅包含一个转移系统。
