Bradley D E
Plasmid. 1985 Mar;13(2):118-28. doi: 10.1016/0147-619x(85)90064-2.
The conjugation systems of three K88-mobilizing plasmids were characterized for the morphology of their pili and type of mating system (surface only or surface + liquid). pREI had a typical IncI1 transfer system with both thick and thin pili. pVIDO determined aggregating thick flexible pili and pPLS nonaggregating thick flexible pili. All three transferred equally well in broth and on plates. pPLS alone was naturally transfer-depressed. pREI and pVIDO were tested for K88 mobilization efficiency, which was greater from their wild-type host strains to Escherichia coli K-12 than between E. coli K-12 strains. The K99 conjugative plasmid from strain B41 was repressed for transfer and determined thick flexible pili that were receptors for the filamentous phage fd.
对三种携带K88的质粒的接合系统进行了特征分析，包括菌毛形态和交配系统类型（仅表面或表面+液体）。pREI具有典型的IncI1转移系统，有粗菌毛和细菌毛。pVIDO产生聚集性的粗而柔韧的菌毛，pPLS产生非聚集性的粗而柔韧的菌毛。这三种质粒在肉汤和平板上的转移效果同样良好。单独的pPLS自然存在转移抑制现象。对pREI和pVIDO进行了K88动员效率测试，结果表明从它们的野生型宿主菌株到大肠杆菌K-12的动员效率高于大肠杆菌K-12菌株之间。来自菌株B41的K99接合质粒的转移受到抑制，并产生了丝状噬菌体fd的受体——粗而柔韧的菌毛。
