Szpeiter Bruno Bernal, Ferreira Juliana Isabel Giuli da Silva, Assis Francisco Flávio Vieira de, Stelmachtchuk Felipe Nascimento, Peixoto Kleber da Cunha, Ajzenberg Daniel, Minervino Antonio Humberto Hammad, Gennari Solange Maria, Marcili Arlei
Universidade Santo Amaro - UNISA, São Paulo, SP, Brasil.
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP, São Paulo, SP, Brasil.
Rev Bras Parasitol Vet. 2017 Apr-Jun;26(2):152-158. doi: 10.1590/S1984-29612017022.
Trypanosoma comprises flagellates able to infect several mammalian species and is transmitted by several groups of invertebrates. The order Chiroptera can be infected by the subgenera Herpetosoma, Schizotrypanum, Megatrypanum and Trypanozoon. In this study, we described the diversity of bat trypanosomes and inferred phylogenetic relationships among the trypanosomes from bats caught in Tapajós-Arapiuns Extractive Reserve (Resex) in Pará state. Trypanosomes from bats were isolated by means of hemoculture, and the molecular phylogeny was based on the trypanosome barcode (SSUrDNA V7V8 variable region). A total of 111 bats were caught in the area, belonging to three families (Emballonuridae, Molossidae and Phyllostomidae) and 12 species. The bat trypanosome prevalence, as evaluated through hemoculture, was 9% all positive cultures were cryopreserve (100% of isolation success). Phylogenetic trees grouped nine isolates in T. cruzi marinkellei branch and only one in T. dionisii branch. Studies on bat trypanosome diversity are important for identifying pathogenic species and for generating support for control measures, especially in such areas where humans inhabit the forest with close contact with bat species. In addition, this is the first study in Resex Tapajós-Arapiuns extractive reserve and further studies should be conducted to elucidate the role of these parasites as environmental degradation biomarkers.
锥虫包括能够感染多种哺乳动物的鞭毛虫，并由几类无脊椎动物传播。翼手目可被赫佩托体属、裂锥虫属、巨锥虫属和锥虫亚属感染。在本研究中，我们描述了蝙蝠锥虫的多样性，并推断了来自帕拉州塔帕若斯-阿拉皮昂斯提取保护区（Resex）捕获的蝙蝠锥虫之间的系统发育关系。通过血液培养分离蝙蝠的锥虫，分子系统发育基于锥虫条形码（SSUrDNA V7V8可变区）。该地区共捕获111只蝙蝠，分属三个科（鞘尾蝠科、犬吻蝠科和叶口蝠科）和12个物种。通过血液培养评估的蝙蝠锥虫患病率为9%，所有阳性培养物均进行了冷冻保存（分离成功率100%）。系统发育树将9个分离株归入克氏锥虫马林凯莱分支，只有1个归入狄氏锥虫分支。对蝙蝠锥虫多样性的研究对于识别致病物种以及为控制措施提供支持非常重要，特别是在人类与蝙蝠物种密切接触的森林居住地区。此外，这是在塔帕若斯-阿拉皮昂斯提取保护区Resex的首次研究，应进一步开展研究以阐明这些寄生虫作为环境退化生物标志物的作用。
