Carmo Ediclei Lima do, Morais Rafaela Dos Anjos Pinheiro Bogoevich, Lima Michele de Souza, Moraes Carla Cristina Guimarães de, Albuquerque George Rêgo, Silva Aristeu Vieira da, Póvoa Marinete Marins
Seção de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, PA, Brasil.
Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários, Universidade Federal do Pará - UFPA, Belém, PA, Brasil.
Rev Bras Parasitol Vet. 2017 Apr-Jun;26(2):226-230. doi: 10.1590/S1984-29612017012.
The relevance of consuming raw or undercooked beef in the transmission of toxoplasmosis is unclear due to the high resistance of cattle to infection. However, this possibility needs to be considered in endemic areas, such as the Amazon, where the consumption of beef is frequent. The objective of this study was to determine the frequency of anti-Toxoplasma gondii IgG antibodies in beef cattle slaughtered in the metropolitan region of Belem, Pará state, Brazil. Blood samples were collected from 500 animals of both genders in a licensed slaughterhouse in Belém. Anti-T. gondii IgG antibodies were detected by an indirect immunofluorescence assay (IFA) with a cut-off titer of 1:64. Anti-T. gondii antibodies were found in 203 animals (40.6%), with a titer of 64 in 112 animals (55.2%), 128 in 68 animals (33.5%), 256 in 15 animals (7.4%), 512 in 5 animals (2.5%), and 1,024 in 3 animals (1.4%). No significant difference was observed between males and females (p > 0.05). The high frequency of anti-T. gondii antibodies observed in beef cattle slaughtered in Belém indicates that the meat of these animals may be an important source of infection for humans and carnivorous domestic animals when inadequately cooked beef is consumed.
由于牛对感染具有高度抗性，食用生牛肉或未煮熟牛肉在弓形虫病传播中的相关性尚不清楚。然而，在牛肉消费频繁的流行地区，如亚马逊地区，需要考虑这种可能性。本研究的目的是确定在巴西帕拉州贝伦市大都市区屠宰的肉牛中抗弓形虫IgG抗体的频率。在贝伦市一家有许可证的屠宰场采集了500头不同性别的动物的血样。采用间接免疫荧光法（IFA）检测抗弓形虫IgG抗体，截断滴度为1:64。在203头动物（40.6%）中检测到抗弓形虫抗体，其中滴度为64的有112头动物（55.2%），128的有68头动物（33.5%），256的有15头动物（7.4%），512的有5头动物（2.5%），1024的有3头动物（1.4%）。雄性和雌性之间未观察到显著差异（p>0.05）。在贝伦市屠宰的肉牛中观察到的抗弓形虫抗体的高频率表明，当食用未充分煮熟的牛肉时，这些动物的肉可能是人类和肉食性家畜感染的重要来源。
