Altmannsberger M, Dirk T, Droese M, Weber K, Osborn M
Virchows Arch B Cell Pathol Incl Mol Pathol. 1986;51(3):265-75. doi: 10.1007/BF02899036.
Monoclonal antibodies which recognize one or only a few keratin polypeptides have been used to study the distribution of different keratins in benign and malignant breast lesions by immunocytochemical methods. Seven monoclonal antibodies which recognized either different keratin polypeptides by immunoblotting techniques, or identified different epithelial cell types in complex tissues were used. In two mastopathies and three fibroadenomas the antibody lu5 stained luminal cells as well as myoepithelial cells. In contrast the antibodies CK7, Troma 1, CK2 and KA4 labeled only luminal cells, whereas antibody CKB1 decorated only myoepithelial cells. All 15 ductal carcinomas showed a uniform staining of tumor cells with the antibodies Troma 1, CK2, KA4 and lu5. The antibody CK7 also stained all ductal carcinomas, but in two specimens the staining was heterogeneous. The antibody CKB1 decorated only the pre-existing myoepithelial cells in 11 of 12 ductal carcinomas but in the remaining specimen the tumor cells were also strongly positive. Tumor cells in lobular carcinomas were labeled by antibodies CK7, Troma 1, CK2, KA4, bu not by CKB1. The antibody CKS1 showed no staining of any of the benign and malignant breast lesions.
识别一种或仅几种角蛋白多肽的单克隆抗体已被用于通过免疫细胞化学方法研究不同角蛋白在乳腺良恶性病变中的分布。使用了七种单克隆抗体，这些抗体要么通过免疫印迹技术识别不同的角蛋白多肽，要么在复杂组织中识别不同的上皮细胞类型。在两个乳腺病和三个纤维腺瘤中，抗体lu5对管腔细胞和肌上皮细胞均有染色。相比之下，抗体CK7、Troma 1、CK2和KA4仅标记管腔细胞，而抗体CKB1仅标记肌上皮细胞。所有15例导管癌的肿瘤细胞经抗体Troma 1、CK2、KA4和lu5染色均呈均匀一致。抗体CK7也对所有导管癌进行了染色，但在两个标本中染色不均匀。抗体CKB1在12例导管癌中的11例中仅标记了预先存在的肌上皮细胞，但在其余标本中肿瘤细胞也呈强阳性。小叶癌中的肿瘤细胞被抗体CK7、Troma 1、CK2、KA4标记，但未被CKB1标记。抗体CKS1对任何乳腺良恶性病变均无染色。
