School of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, 210023, China.
Sci Rep. 2017 Jul 26;7(1):6569. doi: 10.1038/s41598-017-05517-4.
The effects of testosterone and flutamide on reproduction in Brachionus calyciflorus were studied. Asexual reproduction in B. calyciflorus was not affected by testosterone at different concentrations of flutamide. Flutamide in combination with 0, 25, 50, or 75 µg L testosterone had a significant effect on mixis rate. The combination of 5 µg L flutamide with 25 µg L or 50 µg L testosterone resulted in a mixis rate that was 2.2× lower than that with flutamide alone. Fertilization rate was significantly decreased by 7.5 µg L flutamide in combination with 25, 50, or 75 µg L testosterone. The number of resting eggs produced per mictic female was significantly lower at all concentrations of testosterone. A low concentration of flutamide in combination with testosterone resulted in antagonism, increasing the number of resting eggs produced. However, when testosterone was combined with a higher concentration of flutamide, resting egg production declined. Therefore, long-term exposure to either testosterone, flutamide, or a combination of these two compounds may significantly reduce resting egg production in rotifers. This implies that resting egg production is affected differently by hormone pathways.
研究了睾酮和氟他胺对臂尾轮虫生殖的影响。不同浓度氟他胺对臂尾轮虫的无性生殖没有影响。氟他胺与 0、25、50 或 75μg/L 睾酮联合使用对混交率有显著影响。5μg/L 氟他胺与 25μg/L 或 50μg/L 睾酮联合使用导致的混交率比单独使用氟他胺低 2.2 倍。7.5μg/L 氟他胺与 25、50 或 75μg/L 睾酮联合使用，受精率显著降低。在所有睾酮浓度下，产休眠卵的米氏雌性数量均显著降低。低浓度氟他胺与睾酮联合使用会产生拮抗作用，增加休眠卵的产生。然而，当睾酮与较高浓度的氟他胺联合使用时，休眠卵的产生减少。因此，轮虫长期暴露于睾酮、氟他胺或这两种化合物的组合中可能会显著降低休眠卵的产生。这意味着激素途径对休眠卵产生的影响不同。
