Jiangsu Province Key Laboratory for Biodiversity & Biotechnology and Jiangsu Province Key Laboratory for Fisheries Live Food, School of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, PR China.
Jiangsu Province Key Laboratory for Biodiversity & Biotechnology and Jiangsu Province Key Laboratory for Fisheries Live Food, School of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, PR China.
Chemosphere. 2019 Apr;220:146-154. doi: 10.1016/j.chemosphere.2018.12.097. Epub 2018 Dec 15.
Many studies investigated the effects of environmental endocrine disruptors with the rotifer Brachionus calyciflorus. However, they focused on the reproductive behavior of rotifers, especially male-female fertilization, as a parameter in ecotoxicological and endocrine studies. In the present study, we used two environmental hormones (progesterone and testosterone) and one nonsteroidal antiandrogen (flutamide) at five different concentrations (0.5, 1, 2, 4, and 6 mg/L) to study the reproductive behavioral parameters of male rotifers. The average swimming speed of male rotifers in the blank group was 1.14 ± 0.43 mm/s. After exposure for 1 h, testosterone improved the swimming speed of males, with the greatest effect at a concentration of 2-4 mg/L, whereas flutamide and progesterone inhibited the swimming speed. Copulatory behavior experiments showed that, compared with the control group, the recognition ability of males was improved by testosterone at 1, 2, and 3 h (P < 0.05). After 4, 5, and 6 h, progesterone substantially suppressed the mating recognition ability of males, where the density of each group was extremely low at 6 h. Flutamide had a similar effect on the mating recognition ability of male rotifers as that of progesterone. The male fertilization rate in B. calyciflorus increased significantly under testosterone exposure at different concentrations (P < 0.05), with the highest level at 2 mg/L (male fertility rate = 48.61 ± 3.18%). The fertilization rate of male rotifers was suppressed by both progesterone and flutamide (P < 0.05), and higher drug concentrations had stronger suppressive effects.
许多研究都用萼花臂尾轮虫（Brachionus calyciflorus）来研究环境内分泌干扰物的影响。然而，它们主要集中在轮虫的生殖行为上，特别是雄性-雌性受精，作为生态毒理学和内分泌研究的一个参数。在本研究中，我们使用了两种环境激素（孕酮和睾酮）和一种非甾体抗雄激素（氟他胺），在五个不同浓度（0.5、1、2、4 和 6 mg/L）下研究雄性轮虫的生殖行为参数。空白组雄性轮虫的平均游动速度为 1.14 ± 0.43 mm/s。暴露 1 小时后，睾酮提高了雄性的游动速度，在 2-4 mg/L 浓度下效果最大，而氟他胺和孕酮则抑制了游动速度。交配行为实验表明，与对照组相比，睾酮在 1、2 和 3 h 时提高了雄性的识别能力（P < 0.05）。4、5 和 6 h 后，孕酮显著抑制了雄性的交配识别能力，其中 6 h 时每个组的密度极低。氟他胺对雄性轮虫的交配识别能力的影响与孕酮相似。在不同浓度的睾酮暴露下，萼花臂尾轮虫的雄性受精率显著增加（P < 0.05），在 2 mg/L 时达到最高水平（雄性受精率=48.61 ± 3.18%）。孕酮和氟他胺均抑制了雄性轮虫的受精率（P < 0.05），且药物浓度越高抑制作用越强。
