Takahashi K, Matsushima A, Saito Y, Inada Y
Biochem Biophys Res Commun. 1986 Jul 16;138(1):283-8. doi: 10.1016/0006-291x(86)90277-9.
Hemin, having two carboxyl groups, was coupled with monomethoxypolyethylene glycol, PEG, through the ester bond formed with carbodiimide. The PEG-modified hemin was readily soluble not only in neutral aqueous solution but also in organic solvents. Its absorption spectrum in 1,1,1-trichloroethane showed a sharp Soret band at 398 nm. The modified hemin catalyzed the peroxidase-reaction in organic solvent and in aqueous solution using hydrogen peroxide or peroxidized linolenic acid as hydrogen acceptor and o-phenylene diamine as hydrogen donor. The activity of PEG-hemin in 1,1,1-trichloroethane was greater than that in an aqueous solution; k1 values in 1,1,1-trichloroethane were 2.3 X 10(3) M-1 sec-1 with hydrogen peroxide and 7.0 X 10(2) M-1 sec-1 with peroxidized linolenic acid, and the value in an aqueous solution was 3.0 X 10 M-1 sec-1 with hydrogen peroxide.
氯高铁血红素含有两个羧基，通过与碳二亚胺形成的酯键与单甲氧基聚乙二醇（PEG）偶联。PEG修饰的氯高铁血红素不仅易溶于中性水溶液，还易溶于有机溶剂。它在1,1,1-三氯乙烷中的吸收光谱在398nm处显示出尖锐的Soret带。修饰后的氯高铁血红素在有机溶剂和水溶液中，以过氧化氢或过氧化亚麻酸作为氢受体，邻苯二胺作为氢供体，催化过氧化物酶反应。PEG-氯高铁血红素在1,1,1-三氯乙烷中的活性高于在水溶液中的活性；在1,1,1-三氯乙烷中，以过氧化氢为底物时的k1值为2.3×10³M⁻¹s⁻¹，以过氧化亚麻酸为底物时为7.0×10²M⁻¹s⁻¹，而在水溶液中以过氧化氢为底物时的值为3.0×10M⁻¹s⁻¹。
