Harris C Jake, Baulcombe David C
Plant Sciences Department, Cambridge University, Cambridge, UK.
Bio Protoc. 2015 Dec 5;5(23). doi: 10.21769/BioProtoc.1670.
This assay can be used to rapidly and accurately quantify levels of leaf necrosis induced after transient expression of R genes and elicitor combinations (Harris 2013). It is based on the inverse correlation between level of necrosis and chlorophyll content in leaf tissue. It is adapted from the calculations described by (Strain , 1971).
该检测方法可用于快速、准确地定量R基因和激发子组合瞬时表达后诱导的叶片坏死水平（哈里斯，2013年）。它基于叶片组织中坏死水平与叶绿素含量之间的负相关关系。它改编自（斯特林，1971年）所描述的计算方法。
