Release of endogenous aspartate and glutamate induced by electrical stimulation in guinea pig cerebellar slices.

Whether endogenous aspartate and glutamate, candidates for the excitatory neurotransmitter of cerebellar climbing and parallel fibers, are actually released from guinea pig cerebellar slices by electrical stimulation of the cerebellar white matter, was examined by means of mass fragmentography using gas chromatograph-mass spectrometer and thin layer chromatography. Both endogenous aspartate and glutamate were found to be significantly released in a Ca- and stimulus-frequency-dependent manner. Although the origin of each amino acid could not be specified in spite of pharmacological attempt to selectively block the mossy fiber-granule cell (parallel fiber) system, these results were at least in favor of the electrophysiologically and pharmacologically suggested candidacy of these amino acids for the transmitters of cerebellar climbing and parallel fibers.