Phuthong Sophida, Settheetham-Ishida Wannapa, Natphopsuk Sitakan, Settheetham Dariwan, Ishida Takafumi
Department of Physiology, HPV and EBV and Carcinogenesis Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen , Thailand. Email:
Asian Pac J Cancer Prev. 2017 Jul 27;18(7):1815-1819. doi: 10.22034/APJCP.2017.18.7.1815.
Objective: The aim of this study was to investigate the association between genotype and haplotype of MDR1 (C1236T, G2677T/A and C3435T) and the risk for cervical cancer in Northeastern Thai women. Methods: An age-matched case-control study involving squamous cell cervical cancer (SCCA) patients (n=204) and healthy controls (n=204) was enrolled for MDR1 genotyping by real-time PCR method. Results: The genotype distribution of MDR1 in both patients and controls was not significantly different (p>0.05). The haplotype analysis showed that T-T-T was the most common haplotype in this population. Significantly increased risk of cervical cancer was observed in carriers of T-T-C and C-G-T haplotypes with ORs of 1.86 (95%CI=1.02-3.39, p=0.0416) and 2.00 (95%CI=1.18-3.40, p=0.0140), respectively. Analysis of 2677-3435 haplotype showed increased risk for cervical cancer in G-T (OR=1.55; 95% CI=1.12-2.13, p=0.0432) and T-C (OR=1.91; 95%CI=1.05-3.47, p=0.0325). Conclusion: The results provide evidence that haplotype of MDR1 may be an important risk factor for cervical cancer development in Northeastern Thai women.
本研究旨在调查多药耐药基因1（MDR1，C1236T、G2677T/A和C3435T）的基因型和单倍型与泰国东北部女性宫颈癌风险之间的关联。方法：采用实时荧光定量PCR方法对204例鳞状细胞宫颈癌（SCCA）患者和204例健康对照进行年龄匹配的病例对照研究，以对MDR1进行基因分型。结果：患者和对照中MDR1的基因型分布无显著差异（p>0.05）。单倍型分析显示，T-T-T是该人群中最常见的单倍型。观察到携带T-T-C和C-G-T单倍型的个体患宫颈癌的风险显著增加，其比值比分别为1.86（95%可信区间=1.02-3.39，p=0.0416）和2.00（95%可信区间=1.18-3.40，p=0.0140）。对2677-3435单倍型的分析显示，G-T（比值比=1.55；95%可信区间=1.12-2.13，p=0.0432）和T-C（比值比=1.91；95%可信区间=1.05-3.47，p=0.0325）单倍型的个体患宫颈癌的风险增加。结论：结果表明，MDR1单倍型可能是泰国东北部女性宫颈癌发生的一个重要危险因素。
