Larsen Jesper, Sunde Marianne, Islam Md Zohorul, Urdahl Anne Margrete, Barstad Aina Steihaug, Larsen Anders Rhod, Grøntvedt Carl Andreas, Angen Øystein
Statens Serum Institut, Copenhagen S, Denmark.
These authors contributed equally as first authors.
Euro Surveill. 2017 Jul 13;22(28). doi: 10.2807/1560-7917.ES.2017.22.28.30573.
We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.
我们评估了一种广泛使用的基于培养的方法，用于检测从丹麦和挪威的猪以及猪舍内环境中采集的样本中的家畜相关耐甲氧西林金黄色葡萄球菌（LA-MRSA）。在含有头孢西丁和氨曲南的胰蛋白胨大豆肉汤中进行选择性富集导致假阴性结果的比例很高（26%；57/221）。在此基础上，我们建议重新考虑在动物和环境样本中使用选择性富集来检测LA-MRSA。
