Casillas E, Ames W
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1986;84(2):397-400. doi: 10.1016/0742-8413(86)90112-x.
Selected serum parameters (enzyme activities and triglycerides) and liver glutathione and vitamin C concentrations were measured in English sole (Parophrys vetulus) after i.p. injection of carbon tetrachloride (CCl4), a hepatotoxin in fish. Serum lactate dehydrogenase (LDH), alkaline phosphatase (AP) and glutamate dehydrogenase (GDH) activities and the concentration of triglycerides increased in a dose-dependent manner 24 hr post injection. Concentrations of glutathione (reduced and oxidized) and ascorbic acid (vitamin C) in liver did not change in response to CCl4 toxicity 24 hr post injection. These studies indicate that serum AP activity and triglyceride concentrations can be useful in assessing the effects of CCl4-induced liver toxicity in this species of marine fish. Serum LDH and GDH activity should be used with some caution in assessing liver damage in English sole, as other tissues represent more likely sources for serum activity. The levels of liver antioxidants do not appear to be significantly affected, 24 hr post injection, by this particular hepatotoxin.
在腹腔注射四氯化碳（CCl4，一种鱼类肝毒素）后，测定了星斑川鲽（Parophrys vetulus）的选定血清参数（酶活性和甘油三酯）以及肝脏谷胱甘肽和维生素C浓度。注射后24小时，血清乳酸脱氢酶（LDH）、碱性磷酸酶（AP）和谷氨酸脱氢酶（GDH）活性以及甘油三酯浓度呈剂量依赖性增加。注射后24小时，肝脏中谷胱甘肽（还原型和氧化型）和抗坏血酸（维生素C）的浓度并未因CCl4毒性而发生变化。这些研究表明，血清AP活性和甘油三酯浓度可用于评估CCl4诱导的该种海洋鱼类肝脏毒性的影响。在评估星斑川鲽的肝脏损伤时，血清LDH和GDH活性的使用应谨慎，因为其他组织更可能是血清活性的来源。注射后24小时，这种特定的肝毒素似乎并未对肝脏抗氧化剂水平产生显著影响。
