Debbabi Faten, Gargoubi Sondes, Hadj Ayed Mohamed Adnene, Abdessalem Saber Ben
1 Textile Engineering Laboratory, Monastir University, Monastir, Tunisia.
2 Laboratory of chemical, pharmaceutical and pharmacological development of drugs, Faculte de Pharmacie de Monastir, Monastir University, Monastir, Tunisia.
J Biomater Appl. 2017 Sep;32(3):384-398. doi: 10.1177/0885328217721868. Epub 2017 Jul 27.
Braided polyamide sutures are frequently used in dermatologic surgery for wound closure. However, braided sutures promote bacteria proliferation. In order to prevent wound complications due to this effect, antibacterial sutures should be used. The main objective of this study is the development of new non-absorbable antibacterial polyamide braided suture. This paper suggests new coating process that leads to obtain suture uniformly covered by antibacterial film enclosing chitosan, which is known for its antibacterial benefit. Mechanical properties and surface morphology of developed sutures were investigated by using mechanical tests. Sutures surfaces were also examined by scanning electron microscope, to perceive spreading of coating product on suture surface. In order to identify potential reactions between chemical compounds present in coating solution and suture material, sutures were analyzed by ATR-IF spectroscopy. It has been demonstrated that many eventual bonds between compounds present in coating solutions and polyamide macromolecular chain may occur. The existence of these bonds implies the fixation of biopolymer coating on suture surface. It has been demonstrated that uniform surface may be obtained by progressively applying coating solution containing little amount of chitosan on suture surface. We have also found that developed coating process has not affected mechanical properties of suture, which still meet United States Pharmacopeia requirement. Finally, antibacterial effects against four colonies, very widespread in hospitals, were studied. Prominent antibacterial effects of braided polyamide suture against two gram-positive ( S Aureus, S epidermidis) and two gram-negative ( E coli and P aeruginosa) colonies are presented. Optimal result of best properties is obtained by applying three layers of biopolymer coating comprising 1% chitosan and 10% citric acid. The new developed suture coating process appears as a promising method for obtaining important antibacterial effect with smooth suture surface.
编织聚酰胺缝线常用于皮肤科手术中的伤口缝合。然而，编织缝线会促进细菌增殖。为了防止由于这种作用导致的伤口并发症，应使用抗菌缝线。本研究的主要目的是开发新型不可吸收抗菌聚酰胺编织缝线。本文提出了一种新的涂层工艺，该工艺可使缝线均匀地被包裹壳聚糖的抗菌膜覆盖，壳聚糖因其抗菌益处而闻名。通过力学测试研究了所开发缝线的力学性能和表面形态。还通过扫描电子显微镜检查了缝线表面，以观察涂层产品在缝线表面的铺展情况。为了确定涂层溶液中存在的化合物与缝线材料之间的潜在反应，通过ATR-IF光谱对缝线进行了分析。结果表明，涂层溶液中存在的化合物与聚酰胺大分子链之间可能会形成许多最终键。这些键的存在意味着生物聚合物涂层固定在缝线表面。结果表明，通过在缝线表面逐步施加含少量壳聚糖的涂层溶液，可以获得均匀的表面。我们还发现，所开发的涂层工艺并未影响缝线的力学性能，其仍符合美国药典的要求。最后，研究了对医院中非常常见的四种菌落的抗菌效果。展示了编织聚酰胺缝线对两种革兰氏阳性菌（金黄色葡萄球菌、表皮葡萄球菌）和两种革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）菌落的显著抗菌效果。通过施加三层包含1%壳聚糖和10%柠檬酸的生物聚合物涂层，可获得最佳性能的最优结果。新开发的缝线涂层工艺似乎是一种有前景的方法，可获得具有光滑缝线表面的重要抗菌效果。
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