Akhtar Rana Waseem, Shah Syed Aftab Hussain, Qureshi Irfan Zia
Laboratory of Animal and Human Physiology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, 45320 Islamabad, Pakistan.
Laboratory of Animal and Human Physiology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, 45320 Islamabad, Pakistan.
Theriogenology. 2017 Oct 15;102:75-79. doi: 10.1016/j.theriogenology.2017.07.027. Epub 2017 Jul 21.
This study was conducted to determine the response of serum testosterone (T) in male equines (stallions, donkeys and mules) after administering intravenous doses of kisspeptin-10 (KP-10), human chorionic gonadotropin (hCG) and luteinizing hormone (LH) and saline as a control. The animals were divided into four groups of three each: Group I, 3 ml of 0.95% saline; Group II, 50 μg KP-10; Group III, 2500 IU hCG and group IV, 400 μg LH. The administration of KP-10 and hCG to stallions resulted in a significant increase in serum T concentration at 240 min; whereas it was significantly higher at 30, 60, 120, and 240 min with LH treatment as compared to pre-dose concentrations. Both KP-10 and hCG significantly elevated the T concentrations in donkeys at 120 and 240 min, respectively; whereas it was significantly higher at 60, 120, and 240 min with LH treatment as compared to pre-dose concentration. Both KP-10 and LH elevated T in donkeys at 240 min as compared to the control and hCG concentrations. After 120 and 240 min, T concentrations in mules were higher (p < 0.05) with administration of KP-10, hCG and LH as compared to the control. In conclusion, the administration of KP-10, hCG and LH elevate the serum T concentration in normal male equines. It is suggested that KP-10 may be useful in situations where an increase in T is desired. Further work is required to determine the effect of KP-10 on T in male equids with reproductive abnormalities before it can be used in clinical situations.
本研究旨在确定雄性马属动物（种马、驴和骡）静脉注射 kisspeptin-10（KP-10）、人绒毛膜促性腺激素（hCG）和促黄体生成素（LH）以及作为对照的生理盐水后血清睾酮（T）的反应。将动物分为四组，每组三只：第一组，3毫升0.95%生理盐水；第二组，50微克 KP-10；第三组，2500国际单位 hCG；第四组，400微克 LH。给种马注射 KP-10 和 hCG 后，血清 T 浓度在240分钟时显著升高；而与给药前浓度相比，LH 治疗在30、60、120和240分钟时显著更高。KP-10 和 hCG 分别在120和240分钟时显著提高了驴的 T 浓度；而与给药前浓度相比，LH 治疗在60、120和240分钟时显著更高。与对照组和 hCG 浓度相比，KP-10 和 LH 在240分钟时均提高了驴的 T 浓度。在120和240分钟后，与对照组相比，给予 KP-10、hCG 和 LH 的骡的 T 浓度更高（p<0.05）。总之，给予 KP-10、hCG 和 LH 可提高正常雄性马属动物的血清 T 浓度。建议在需要提高 T 水平的情况下，KP-10 可能有用。在将 KP-10 用于临床情况之前，需要进一步研究以确定其对患有生殖异常的雄性马科动物 T 的影响。
