Kolesnik Ekaterina, Obiegala Anna, Marschang Rachel E
Laboklin GmbH & Co. KG, Bad Kissingen, Germany (Kolesnik, Marschang).
Institut of Animal Hygiene, University of Leipzig, Leipzig, Germany (Obiegala).
J Vet Diagn Invest. 2017 Nov;29(6):820-832. doi: 10.1177/1040638717722387. Epub 2017 Jul 28.
We tested samples from 1,015 chelonians in Europe for Mycoplasma spp., herpesviruses, ranaviruses, picornaviruses, and ferlaviruses by PCR. Mycoplasma spp. were detected in 42.1% and herpesviruses were detected in 8.0% of tested chelonians. Differentiation of the herpesviruses revealed that 46.9% of the detected chelonian viruses were testudinid herpesvirus 1 (TeHV-1) and 54.3% were TeHV-3, including co-detections of TeHV-1 and -3 in 3 tortoises. TeHV-4 was detected in a leopard tortoise ( Stigmochelys pardalis), and a herpesvirus that could not be further characterized was found in a pond slider ( Trachemys scripta). Picornaviruses (topiviruses) were detected in 2.2% of the tested animals; ferlaviruses were found in 0.6%; no ranaviruses were detected in any of the animals tested. Mycoplasma spp. were detected significantly more often in Horsfield's tortoises ( Testudo horsfieldii), leopard tortoises, and Indian star tortoises ( Geochelone elegans) than in other species. Horsfield's tortoises were also significantly more often positive for TeHV-1. Mycoplasma and TeHV-1 were co-detected in 3.0%, and mycoplasma and TeHV-3 in 2.3%. The TeHV-4-positive tortoise was also positive for mycoplasma. Mycoplasma and picornaviruses were co-detected in 1.2% of the tortoises. A spur-thighed tortoise ( Testudo graeca) was positive for mycoplasma and a ferlavirus. In some cases, >2 pathogens were detected. A significant correlation between mycoplasma and herpesvirus detection was found. Of all tested animals, 47.6% were positive for at least one pathogen, demonstrating the importance of pathogen detection in captive chelonians.
我们通过聚合酶链反应（PCR）对欧洲1015只龟类的样本进行了支原体属、疱疹病毒、蛙病毒、小核糖核酸病毒和费拉病毒检测。在42.1%的受测龟类中检测到支原体属，在8.0%的受测龟类中检测到疱疹病毒。对疱疹病毒的鉴别显示，检测到的龟类病毒中有46.9%是陆龟疱疹病毒1型（TeHV-1），54.3%是TeHV-3，其中3只陆龟同时检测到TeHV-1和TeHV-3。在一只豹纹陆龟（Stigmochelys pardalis）中检测到TeHV-4，在一只红耳龟（Trachemys scripta）中发现了一种无法进一步鉴定的疱疹病毒。在2.2%的受测动物中检测到小核糖核酸病毒（topiviruses）；在0.6%的动物中发现了费拉病毒；在所有受测动物中均未检测到蛙病毒。在四爪陆龟（Testudo horsfieldii）、豹纹陆龟和印度星龟（Geochelone elegans）中检测到支原体属的频率显著高于其他物种。四爪陆龟中TeHV-1呈阳性的频率也显著更高。支原体属和TeHV-1同时被检测到的比例为3.0%，支原体属和TeHV-3同时被检测到的比例为2.3%。TeHV-4阳性的陆龟支原体属检测也呈阳性。在1.2%的陆龟中同时检测到支原体属和小核糖核酸病毒。一只希腊陆龟（Testudo graeca）支原体属和费拉病毒检测呈阳性。在某些情况下，检测到了两种以上的病原体。发现支原体属和疱疹病毒检测之间存在显著相关性。在所有受测动物中，47.6%至少有一种病原体检测呈阳性，这表明在圈养龟类中进行病原体检测的重要性。
