在印度一家医院实验室对用于结核样本处理和液体培养的OMNIgene®•痰液产品进行评估。

Evaluation of OMNIgene®•SPUTUM for tuberculosis sample processing and liquid culture at a hospital laboratory in India.

作者信息

Nambiar Remya, Shaw Kaitlyn, Curry Patricia S

机构信息

Microbiology Section, Department of Laboratory Medicine, PD Hinduja Hospital and Medical Research Centre, Floor 5, Lalita Giridhar (S1) Building, Off Veer Savarkar Marg, Mahim (West), Mumbai 400016, India.

DNA Genotek, 3000 - 500 Palladium Drive, Ottawa, Ontario K2V 1C2, Canada.

出版信息

J Microbiol Methods. 2017 Oct;141:60-62. doi: 10.1016/j.mimet.2017.07.013. Epub 2017 Jul 25.

DOI:10.1016/j.mimet.2017.07.013

PMID:28754448

Abstract

This preliminary evaluation examined the reagent OMNIgene®•SPUTUM (OM-S) as a tool to eliminate NaOH/NALC processing prior to Middlebrook liquid culture for Mycobacterium tuberculosis (MTb). Twenty-seven manually split samples (OM-S-treated vs. NaOH/NALC) showed 100% agreement: 81.5% MTb-positive and 18.5% MTb-negative. On average, OM-S-treated specimens required 1.2 additional days to culture positivity.

摘要

这项初步评估将OMNIgene®•SPUTUM（OM-S）试剂作为一种工具，用于在结核分枝杆菌（MTb）的Middlebrook液体培养之前消除NaOH/NALC处理。27个手动分割样本（OM-S处理组与NaOH/NALC处理组）显示出100%的一致性：81.5%为MTb阳性，18.5%为MTb阴性。平均而言，经OM-S处理的标本培养出阳性结果需要多1.2天。