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全氟烷基物质在人骨中的含量:对骨细胞分化的影响

Perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation.

机构信息

Institute of Cancer Research and Translational Medicine, Department of Anatomy and Cell Biology, Faculty of Medicine, University of Oulu, Oulu, Finland.

National Institute for Health and Welfare, Chemicals and Health Unit, Kuopio, Finland.

出版信息

Sci Rep. 2017 Jul 28;7(1):6841. doi: 10.1038/s41598-017-07359-6.

Abstract

Perfluoroalkyl substances (PFAS), including two most commonly studied compounds perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), are widely distributed environmental pollutants, used extensively earlier. Due to their toxicological effects the use of PFAS is now regulated. Based on earlier studies on PFOA's distribution in bone and bone marrow in mice, we investigated PFAS levels and their possible link to bone microarchitecture of human femoral bone samples (n = 18). Soft tissue and bone biopsies were also taken from a 49-year old female cadaver for PFAS analyses. We also studied how PFOA exposure affects differentiation of human osteoblasts and osteoclasts. PFAS were detectable from all dry bone and bone marrow samples, PFOS and PFOA being the most prominent. In cadaver biopsies, lungs and liver contained the highest concentrations of PFAS, whereas PFAS were absent in bone marrow. Perfluorononanoic acid (PFNA) was present in the bones, PFOA and PFOS were absent. In vitro results showed no disturbance in osteogenic differentiation after PFOA exposure, but in osteoclasts, lower concentrations led to increased resorption, which eventually dropped to zero after increase in PFOA concentration. In conclusion, PFAS are present in bone and have the potential to affect human bone cells partly at environmentally relevant concentrations.

摘要

全氟烷基物质(PFAS)包括两种最常研究的化合物全氟辛烷磺酸(PFOS)和全氟辛酸(PFOA),是广泛分布的环境污染物,早期广泛使用。由于其毒理学效应,PFAS 的使用现在受到监管。基于先前关于 PFOA 在小鼠骨骼和骨髓中分布的研究,我们研究了 PFAS 水平及其与人类股骨骨样本来的骨微结构的可能联系(n=18)。还从一名 49 岁的女性尸体上采集软组织和骨活检用于 PFAS 分析。我们还研究了 PFOA 暴露如何影响人成骨细胞和破骨细胞的分化。所有干骨和骨髓样本均能检测到 PFAS,其中 PFOS 和 PFOA 最为突出。在尸检活检中,肺和肝中 PFAS 的浓度最高,而骨髓中则没有。全氟壬酸(PFNA)存在于骨骼中,而 PFOA 和 PFOS 则不存在。体外结果显示,PFOA 暴露后成骨分化没有受到干扰,但在破骨细胞中,较低浓度导致吸收增加,在 PFOA 浓度增加后最终降至零。总之,PFAS 存在于骨骼中,在环境相关浓度下有可能影响人类骨细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/173e/5533791/131799afcf5f/41598_2017_7359_Fig1_HTML.jpg

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