Saleh Amgad A, Sharafaddin Anwar H, El-Komy Mahmoud H, Ibrahim Yasser E, Hamad Younis K, Molan Younis Y
Faculty of Food and Agriculture Sciences, Department of Plant Protection, King Saud University, Box 2460, Riyadh, 11451, Saudi Arabia.
Plant Pathology Institute, Agriculture Research Center, Giza, Egypt.
Methods Mol Biol. 2017;1638:273-282. doi: 10.1007/978-1-4939-7159-6_22.
Bayoud disease, caused by Fusarium oxysporum f. sp. albedinis, is a very serious and destructive disease to date palm. Screening of date palm germplasm for resistance to bayoud disease is a crucial step to avoid or alleviate the disease consequences. Fortunately, it was discovered that there are two mitochondrial plasmid-like DNA molecules associated with susceptibility or resistance to bayoud disease. In this chapter, we present a fast, simple, and reliable technique to screen date palm germplasm for the presence of these mitochondrial molecular markers associated with susceptibility or resistance to bayoud.
由尖孢镰刀菌枣椰专化型引起的枣椰树枯萎病,是迄今为止对枣椰树极具严重性和毁灭性的病害。筛选抗枣椰树枯萎病的枣椰树种质是避免或减轻该病害后果的关键步骤。幸运的是,人们发现有两个类线粒体质粒DNA分子与枣椰树枯萎病的感病性或抗性相关。在本章中,我们介绍一种快速、简便且可靠的技术,用于筛选枣椰树种质中这些与枣椰树枯萎病感病性或抗性相关的线粒体分子标记。